RRC ID 36357
Author Ishii M, Matsumoto Y, Sekimizu K.
Title Usefulness of silkworm as a host animal for understanding pathogenicity of Cryptococcus neoformans.
Journal Drug Discov Ther
Abstract We propose Cryptococcus neoformans infection model using silkworm for understanding cryptococcosis and screening of therapeutically effective antibiotics. Silkworm is an insect whose rearing methods were established through a long history of the sericulture industry. Silkworm facilitates experiments using a large number of individuals because of low cost for rearing and few ethical problems caused by killing animals. Silkworm can be reared at 37˚C to perform infection experiments at same temperature to human body. Injection of accurate amounts of samples into hemolymph of silkworm by usual syringes is easy to be done since silkworm has an appropriate size to handle. Moreover two injection methods, injection into hemolymph and intestine, are distinguishable for silkworms. The former is correspondent to intravenous injection, and the latter is to oral administration in humans. Taking these advantages of silkworms as host animals, it is possible to evaluate the virulence factors in C. neoformans and the therapeutic efficacy of antifungal agents.
Volume 10(1)
Pages 9-13
Published 2016-2
DOI 10.5582/ddt.2016.01015
PMID 26902902
MeSH Animals Anti-Bacterial Agents / pharmacology Bombyx / drug effects Bombyx / embryology Bombyx / microbiology* Cryptococcosis / drug therapy Cryptococcosis / microbiology* Cryptococcus neoformans / drug effects Cryptococcus neoformans / pathogenicity* Disease Models, Animal Drug Discovery / methods Humans Larva Species Specificity Virulence