RRC ID 36370
Author Uno T, Furutani M, Watanabe C, Sakamoto K, Uno Y, Kanamaru K, Yamagata H, Mizoguchi A, Takeda M.
Title Rab proteins in the brain and corpus allatum of Bombyx mori.
Journal Histochem Cell Biol
Abstract In eukaryotic cells, Rab guanosine triphosphate-ases serve as key regulators of membrane-trafficking events, such as exocytosis and endocytosis. Rab3, Rab6, and Rab27 control the regulatory secretory pathway of neuropeptides and neurotransmitters. The cDNAs of Rab3, Rab6, and Rab27 from B. mori were inserted into a plasmid, transformed into Escherichia coli, and then subsequently purified. We then produced antibodies against Rab3, Rab6, and Rab27 of Bombyx mori in rabbits and rats for use in western immunoblotting and immunohistochemistry. Western immunoblotting of brain tissue revealed a single band at approximately 26 kDa. Immunohistochemistry results revealed that Rab3, Rab6, and Rab27 expression was restricted to neurons in the pars intercerebralis and dorsolateral protocerebrum of the brain. Rab3 and Rab6 co-localized with bombyxin, an insect neuropeptide. However, there was no Rab that co-localized with prothoracicotropic hormone. The corpus allatum secretes neuropeptides synthesized in the brain into the hemolymph. Results showed that Rab3 and Rab6 co-localized with bombyxin in the corpus allatum. These findings suggest that Rab3 and Rab6 are involved in neurosecretion in B. mori. This study is the first to report a possible relationship between Rab and neurosecretion in the insect corpus allatum.
Volume 146(1)
Pages 59-69
Published 2016-7-1
DOI 10.1007/s00418-016-1422-y
PII 10.1007/s00418-016-1422-y
PMID 26976000
MeSH Animals Antibodies / immunology Bombyx / chemistry* Bombyx / immunology Brain / immunology* Corpora Allata / chemistry* Corpora Allata / immunology* Immunohistochemistry Rabbits Rats rab GTP-Binding Proteins / analysis* rab GTP-Binding Proteins / immunology
IF 3.418
Times Cited 3