RRC ID 36501
Author Himi E, Taketa S.
Title Barley Ant17, encoding flavanone 3-hydroxylase (F3H), is a promising target locus for attaining anthocyanin/proanthocyanidin-free plants without pleiotropic reduction of grain dormancy.
Journal Genome
Abstract Preharvest sprouting is a serious problem in grain crop production because it causes quality deterioration and economic losses. It is well known that grain colour is closely associated with grain dormancy in wheat; white-grained lines without accumulating proanthocyanidins in testa tend to be more susceptible to preharvest sprouting than red ones. All available white-grained wheat lines are restricted to triple recessive mutations at the R loci (R-A1, R-B1, and R-D1), but barley is known to have 11 independent loci conferring the proanthocyanidin-free grain phenotype. In this study, we evaluated the dormancy levels of anthocyanin/proanthocyanidin-free ant17 mutants. Three ant17 mutants showed the same levels of dormancy as their respective wild types. Sequencing of three independent ant17 alleles detected a point mutation within the coding regions of flavanone-3-hydroxylase (F3H), which are predicted to cause a premature stop codon at different sites. The F3H locus completely cosegregated with the Ant17 position on the chromosome arm 2HL. Expression of the barley F3H gene was observed in pigmented tissues, but not in nonpigmented roots and stems. This result indicates that wheat F3H may be a promising new target locus for breeding white-grained lines with a practical level of preharvest sprouting resistance.
Volume 58(1)
Pages 43-53
Published 2015-1-1
DOI 10.1139/gen-2014-0189
PMID 25932661
MeSH Anthocyanins / metabolism* Chromosome Mapping Chromosomes, Plant / genetics Germination Hordeum / enzymology Hordeum / physiology* Mixed Function Oxygenases / genetics* Mixed Function Oxygenases / metabolism Plant Dormancy Plant Proteins / genetics* Plant Proteins / metabolism Point Mutation Quantitative Trait Loci Seeds / genetics Seeds / growth & development
IF 2.037
Times Cited 4
Barley Barley seed samples