Reference - Detail
| RRC ID | 36505 |
|---|---|
| Author | Bao LQ, Huy NT, Kikuchi M, Yanagi T, Senba M, Shuaibu MN, Honma K, Yui K, Hirayama K. |
| Title | CD19(+) B cells confer protection against experimental cerebral malaria in semi-immune rodent model. |
| Journal | PLoS One |
| Abstract |
In African endemic area, adults are less vulnerable to cerebral malaria than children probably because of acquired partial immunity or semi-immune status. Here, we developed an experimental cerebral malaria (ECM) model for semi-immune mice. C57BL/6 (B6) mice underwent one, two and three cycles of infection and radical treatment (1-cure, 2-cure and 3-cure, respectively) before being finally challenged with 10(4) Plasmodium berghei ANKA without treatment. Our results showed that 100% of naïve (0-cure), 67% of 1-cure, 37% of 2-cure and none of 3-cure mice succumbed to ECM within 10 days post challenge infection. In the protected 3-cure mice, significantly higher levels of plasma IL-10 and lower levels of IFN-γ than the others on day 7 post challenge infection were observed. Major increased lymphocyte subset of IL-10 positive cells in 3-cure mice was CD5(-)CD19(+) B cells. Passive transfer of splenic CD19(+) cells from 3-cure mice protected naïve mice from ECM. Additionally, aged 3-cure mice were also protected from ECM 12 and 20 months after the last challenge infection. In conclusion, mice became completely resistant to ECM after three exposures to malaria. CD19(+) B cells are determinants in protective mechanism of semi-immune mice against ECM possibly via modulatory IL-10 for pathogenic IFN-γ production. |
| Volume | 8(5) |
| Pages | e64836 |
| Published | 2013-1-1 |
| DOI | 10.1371/journal.pone.0064836 |
| PII | PONE-D-12-36336 |
| PMID | 23724100 |
| PMC | PMC3665539 |
| MeSH | Adoptive Transfer Animals Antibodies, Protozoan / blood Antigens, CD19 / metabolism* B-Lymphocytes / immunology* CD5 Antigens / metabolism Disease Models, Animal Disease Resistance / immunology Disease Susceptibility Immunoglobulin G / blood Interferon-gamma / blood Interleukin-10 / biosynthesis Interleukin-10 / blood Malaria, Cerebral / blood Malaria, Cerebral / immunology* Malaria, Cerebral / parasitology Malaria, Cerebral / prevention & control* Mice Mice, Inbred C57BL Parasitemia / immunology Parasitemia / parasitology Plasmodium berghei / physiology Species Specificity Spleen / pathology |
| IF | 2.74 |
| Times Cited | 9 |
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| Total number of mentions | 0 |
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