論文 - 詳細
| RRC ID | 36660 |
|---|---|
| 著者 | Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S. |
| タイトル | A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. |
| ジャーナル | Sci Rep |
| Abstract |
In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system. |
| 巻・号 | 4 |
| ページ | 3594 |
| 公開日 | 2014-1-8 |
| DOI | 10.1038/srep03594 |
| PII | srep03594 |
| PMID | 24399248 |
| PMC | PMC3884228 |
| MeSH | Cell Culture Techniques Cell Differentiation Culture Media Humans Induced Pluripotent Stem Cells / cytology* Serial Passage |
| IF | 3.998 |
| 引用数 | 227 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 100 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 研究用ヒト臍帯血幹細胞 | 研究用ヒト臍帯血幹細胞 |