RRC ID 36660
Author Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.
Title A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.
Journal Sci Rep
Abstract In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system.
Volume 4
Pages 3594
Published 2014-1-8
DOI 10.1038/srep03594
PII srep03594
PMID 24399248
PMC PMC3884228
MeSH Cell Culture Techniques Cell Differentiation Culture Media Humans Induced Pluripotent Stem Cells / cytology* Serial Passage
IF 4.259
Times Cited 81
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Cord blood stem cells for research 研究用ヒト臍帯血幹細胞