RRC ID 36664
著者 Ohshima S, Mori S, Shigenari A, Miyamoto A, Takasu M, Imaeda N, Nunomura S, Okayama Y, Tanaka M, Kitagawa H, Kulski JK, Inoko H, Ando A, Kametani Y.
タイトル Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody.
ジャーナル Biosci Trends
Abstract CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells with a likely role in cell survival, proliferation and differentiation. In order to study the differentiation activity of porcine CD117 hematopoietic cells in vitro and in vivo we prepared an anti-swine CD117 Mab (2A1) with high specificity for flow-cytometrical analysis. The 2A1 Mab did not recognize mouse or human mast cells suggesting that 2A1 is species-specific. Swine bone marrow (BM) CD117+ cells differentiated in vitro mainly into erythroid and monocyte lineages in the methylcellulose-based colony assay. When the swine BM CD117+ cells were transplanted in vivo into immunodeficient NOG (NOD/SCID/IL-2gc-null) mice, a significant amount of swine CD45+ leukocytes, including CD3 positive T cells, were developed in the mice. These results revealed that the swine BM CD117+ cells possess hematopoietic stem/progenitor activity and when monitored in immunodeficient mice or in vitro they can develop into lymphoid, erythroid, and myeloid cells efficiently with the new monoclonal antibody.
巻・号 8(6)
ページ 308-15
公開日 2014-12-1
DOI 10.5582/bst.2014.01084
PMID 25641176
MeSH Animals Antibodies, Monoclonal* Cell Differentiation / physiology* Cell Separation / methods* Flow Cytometry Hematopoietic Stem Cells / physiology* Mice Multipotent Stem Cells / physiology* Proto-Oncogene Proteins c-kit / immunology Species Specificity Swine
IF 1.553
引用数 1
WOS 分野 BIOLOGY
リソース情報
研究用ヒト臍帯血幹細胞 研究用ヒト臍帯血幹細胞