| RRC ID |
3668
|
| Author |
You M, Nishiguchi T, Saito A, Isawa T, Mitsui H, Minamisawa K.
|
| Title |
Expression of the nifH gene of a Herbaspirillum endophyte in wild rice species: daily rhythm during the light-dark cycle.
|
| Journal |
Appl Environ Microbiol
|
| Abstract |
The expression of nitrogenase genes of Herbaspirillum sp. B501 associated in shoot (leaf and stem) of wild rice, Oryza officinalis, was studied by means of reverse transcription-PCR (RT-PCR) targeted at the nifH gene. RT-PCR analyses indicate that nifH transcript was detected exclusively from nitrogen-fixing cells of gfp-tagged strain B501gfp1 in both free-living and endophytic states by using a constitutive gfp gene transcript as a positive control. Transcription of nifH and nitrogen fixation in free-living cells were induced maximally at a 2% O2 concentration and repressed in free air (21% O2). nifH transcription was monitored in the endophytic cells by using total RNA extracted from B501gfp1-inoculated wild rice plants during daily light-dark cycles. The level of nifH transcription in planta varied dramatically, with a maximum during the light period. Moreover, the light radiation enhanced nifH expression even in free-living cells grown in culture. These results suggest that in planta nitrogen fixation by the endophyte shows a daily rhythm determined by the plant's light environment.
|
| Volume |
71(12)
|
| Pages |
8183-90
|
| Published |
2005-12-1
|
| DOI |
10.1128/AEM.71.12.8183-8190.2005
|
| PII |
71/12/8183
|
| PMID |
16332801
|
| PMC |
PMC1317309
|
| MeSH |
Amino Acid Sequence
Base Sequence
Biological Clocks
Circadian Rhythm
Consensus Sequence
DNA Primers
Darkness
Herbaspirillum / enzymology*
Herbaspirillum / growth & development
Herbaspirillum / radiation effects
Light
Molecular Sequence Data
Oryza / microbiology*
Oxidoreductases / chemistry
Oxidoreductases / genetics*
Oxidoreductases / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Transcription, Genetic
|
| IF |
4.016
|
| Times Cited |
35
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| Resource |
| Rice |
W0012 |
