RRC ID 36743
Author Uhrig RG, Labandera AM, Muhammad J, Samuel M, Moorhead GB.
Title Rhizobiales-like Phosphatase 2 from Arabidopsis thaliana Is a Novel Phospho-tyrosine-specific Phospho-protein Phosphatase (PPP) Family Protein Phosphatase.
Journal J Biol Chem
Abstract Cellular signaling through protein tyrosine phosphorylation is well established in mammalian cells. Although lacking the classic tyrosine kinases present in humans, plants have a tyrosine phospho-proteome that rivals human cells. Here we report a novel plant tyrosine phosphatase from Arabidopsis thaliana (AtRLPH2) that, surprisingly, has the sequence hallmarks of a phospho-serine/threonine phosphatase belonging to the PPP family. Rhizobiales/Rhodobacterales/Rhodospirillaceae-like phosphatases (RLPHs) are conserved in plants and several other eukaryotes, but not in animals. We demonstrate that AtRLPH2 is localized to the plant cell cytosol, is resistant to the classic serine/threonine phosphatase inhibitors okadaic acid and microcystin, but is inhibited by the tyrosine phosphatase inhibitor orthovanadate and is particularly sensitive to inhibition by the adenylates, ATP and ADP. AtRLPH2 displays remarkable selectivity toward tyrosine-phosphorylated peptides versus serine/threonine phospho-peptides and readily dephosphorylates a classic tyrosine phosphatase protein substrate, suggesting that in vivo it is a tyrosine phosphatase. To date, only one other tyrosine phosphatase is known in plants; thus AtRLPH2 represents one of the missing pieces in the plant tyrosine phosphatase repertoire and supports the concept of protein tyrosine phosphorylation as a key regulatory event in plants.
Volume 291(11)
Pages 5926-5934
Published 2016-3-11
DOI 10.1074/jbc.M115.683656
PII S0021-9258(20)42832-7
PMID 26742850
PMC PMC4786726
MeSH Adenosine Diphosphate / metabolism Adenosine Triphosphate / metabolism Arabidopsis / chemistry Arabidopsis / cytology Arabidopsis / metabolism* Arabidopsis Proteins / analysis Arabidopsis Proteins / metabolism* Phosphoprotein Phosphatases / analysis Phosphoprotein Phosphatases / metabolism* Phosphorylation Protein Tyrosine Phosphatases / analysis Protein Tyrosine Phosphatases / metabolism
IF 4.238
Times Cited 5
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pst15793 pst16212