RRC ID 36744
Author Kataya AR, Schei E, Lillo C.
Title Towards understanding peroxisomal phosphoregulation in Arabidopsis thaliana.
Journal Planta
Abstract MAIN CONCLUSION:This work identifies new protein phosphatases and phosphatase-related proteins targeting peroxisomes, and raises the question of a novel protein import pathway from ER to peroxisomes involving peroxisomal targeting signal type 1 (PTS1) Plant peroxisomes are essential for several processes, for example lipid metabolism, free radical detoxification, development, and stress-related functions. Although research on peroxisomes has been intensified, reversible phosphorylation as a control mechanism in peroxisomes is barely studied. Therefore, it is crucial to identify all peroxisomal proteins involved in phosphoregulation. We here started with protein phosphatases, and searched the Arabidopsis thaliana genome for phosphatase-related proteins with putative peroxisomal targeting signals (PTS). Five potential peroxisomal candidates were detected, from which four were confirmed to target peroxisomes or have a functional PTS. The highly conserved Ser-Ser-Met> was validated for two protein phosphatase 2C (PP2C) family members (POL like phosphatases, PLL2 and PLL3) as a functional peroxisomal targeting signal type 1 (PTS1). Full-length PLL2 and PLL3 fused with a reporter protein targeted peroxisomes in two plant expression systems. A putative protein phosphatase, purple acid phosphatase 7 (PAP7), was found to be dually targeted to ER and peroxisomes and experiments indicated a possible trafficking to peroxisomes via the ER depending on peroxisomal PTS1. In addition, a protein phosphatase 2A regulator (TIP41) was validated to harbor a functional PTS1 (Ser-Lys-Val>), but the full-length protein targeted cytosol and nucleus. Reverse genetics indicated a role for TIP41 in senescence signaling. Mass spectrometry of whole seedlings and isolated peroxisomes was employed, and identified new putative phosphorylated peroxisomal proteins. Previously, only one protein phosphatase, belonging to the phospho-protein phosphatase (PPP) family, was identified as a peroxisomal protein. The present work implies that members of two other main protein phosphatase families, i.e. PP2C and PAP, are also targeting peroxisomes.
Volume 243(3)
Pages 699-717
Published 2016-3-1
DOI 10.1007/s00425-015-2439-5
PII 10.1007/s00425-015-2439-5
PMID 26649560
MeSH Acid Phosphatase / genetics Acid Phosphatase / metabolism Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cytosol / metabolism Endoplasmic Reticulum / metabolism Gene Expression Regulation, Plant Genome, Plant / genetics* Glycoproteins / genetics Glycoproteins / metabolism Peroxisomes / enzymology* Phosphorylation Protein Phosphatase 2C / genetics Protein Phosphatase 2C / metabolism Protein Sorting Signals / genetics Protein Transport Seedlings / enzymology Seedlings / genetics
IF 3.06
Times Cited 7
Arabidopsis / Cultured plant cells, genes pda07225