Reference - Detail
| RRC ID | 36762 |
|---|---|
| Author | Izumi G, Koga K, Nagai M, Urata Y, Takamura M, Harada M, Hirata T, Hirota Y, Ogawa K, Inoue S, Fujii T, Osuga Y. |
| Title | Cyclic stretch augments production of neutrophil chemokines, matrix metalloproteinases, and activin a in human endometrial stromal cells. |
| Journal | Am J Reprod Immunol |
| Abstract |
PROBLEM:The aim of this study was to test our hypothesis: Contractile activity that occurs in the uterus during menstruation induces biochemical factors that enhance remodeling of the endometrium. METHOD OF STUDY:Cyclic stretch, mimicking contractile activity during menstruation, was applied to human endometrial stromal cells (ESC) using the Flexercell Tension system. The concentration and activity of CXCL8, CXCL1, MMPs, and activin A were measured using ELISAs and specific assays. Neutrophil chemotactic activity was evaluated using migration assays. RESULTS:Cyclic stretch significantly induced ESC secretion of CXCL8 and CXCL1 and neutrophil chemotaxis. Stretch also increased MMP-1, MMP-2, and MMP-3 activity, activin A secretion, and activity in ESC. CONCLUSION:These results indicate that the contractile activities of the uterus during menstruation contribute to the remodeling of the endometrium, by inducing chemokine secretion, MMP expression, activity, and neutrophil chemotaxis. |
| Volume | 73(6) |
| Pages | 501-6 |
| Published | 2015-6-1 |
| DOI | 10.1111/aji.12359 |
| PMID | 25605062 |
| MeSH | Activins / immunology* Chemokine CXCL11 / immunology* Collagenases / immunology* Endometrium / immunology* Female Humans Interleukin-8 / immunology* Menstruation / immunology* Neutrophils / immunology* Stromal Cells / immunology Uterine Contraction / immunology |
| IF | 2.739 |
| Times Cited | 4 |
| WOS Category | REPRODUCTIVE BIOLOGY IMMUNOLOGY |
| Resource | |
| Human and Animal Cells | F5-5.fl(RCB0560) |