RRC ID 36778
著者 Miyazaki J, Tsurui H, Kawasumi K, Kobayashi T.
タイトル Sensitivity enhancement of photothermal microscopy with radially segmented balanced detection.
ジャーナル Opt Lett
Abstract A novel detection method is proposed for highly sensitive photothermal microscopic imaging. This method is based on the characteristics of an angular-dependent photothermal signal; it improves signal intensity by up to two times and rejects the intensity noise of the probe beam. The subdiffraction resolution photothermal imaging of mouse skin melanoma is demonstrated using a laser diode-based photothermal microscopy system to evaluate this method. We confirm that the signal intensity is enhanced 1.7 times compared with the conventional detection method. Moreover, the intensity noise of the laser diode used for the probe beam is effectively reduced by approximately 31 dB, even for a sample with non-uniformity of the refractive index and stationary absorption. This method is implemented by means of a commonly used balanced detector and is thus potentially useful for high-speed imaging.
巻・号 40(4)
ページ 479-82
公開日 2015-2-15
DOI 10.1364/OL.40.000479
PII 311667
PMID 25680129
MeSH Animals Image Processing, Computer-Assisted* Melanoma, Experimental / pathology Mice Microscopy / methods* Optical Phenomena* Signal-To-Noise Ratio
IF 3.714
引用数 11
WOS 分野 OPTICS
リソース情報
ヒト・動物細胞 B16 melanoma(RCB1283)