RRC ID 36783
Author Kato H, Ochiai-Shino H, Onodera S, Saito A, Shibahara T, Azuma T.
Title Promoting effect of 1,25(OH)2 vitamin D3 in osteogenic differentiation from induced pluripotent stem cells to osteocyte-like cells.
Journal Open Biol
Abstract We recently reported a new method to purify the induced pluripotent stem (iPS)-derived osteoprogenitors (iPSop). In this paper, we optimized the procedure and characterized cells at each process step. We observed that 10 days of treatment with FGF-2, IGF-1 and TGF-β (FIT) resulted in early-phase osteoblasts and 14 days of treatment resulted in late-phase osteoblasts. We found that treatment with 1,25(OH)2 vitamin D3 increased expression of osteocalcin and decreased expression of tissue-non-specific alkaline phosphatase and runt-related transcription factor 2 (RUNX2) in iPSop-day14 cells (cells treated with FIT for 14 days). Therefore, iPSop-day14 cells were promoted to mature osteoblasts by 1,25(OH)2 vitamin D3 treatment. In addition, we found that 1,25(OH)2 vitamin D3 treatment for 14 days enhanced not only mineralization but also expression of osteocyte markers, including dentin matrix protein-1 and fibroblast growth factor-23, in iPSop cells. Therefore, 1,25(OH)2 vitamin D3 is a potent promoter of osteoblast-osteocyte transition. The results of this study suggest that it is possible to evaluate both early- and late-phase osteoblasts and to apply cells to drug screening for anabolic drugs that stimulate bone formation.
Volume 5(2)
Pages 140201
Published 2015-2
DOI 10.1098/rsob.140201
PII rsob.140201
PMID 25652541
PMC PMC4345281
MeSH Animals Biomarkers Calcitriol / pharmacology* Cell Differentiation / drug effects* Cell Differentiation / genetics Cell Line Embryoid Bodies / metabolism Gene Expression Regulation Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects* Induced Pluripotent Stem Cells / metabolism Mice Osteocytes / cytology* Osteocytes / drug effects* Osteogenesis / drug effects* Osteogenesis / genetics
IF 3.286
Times Cited 7
Human and Animal Cells 201B7(HPS0063)