| RRC ID |
36793
|
| Author |
Kagawa Y, Matsuura K, Shimizu T, Tsuneda S.
|
| Title |
Direct measurement of local dissolved oxygen concentration spatial profiles in a cell culture environment.
|
| Journal |
Biotechnol Bioeng
|
| Abstract |
Controlling local dissolved oxygen concentration (DO) in media is critical for cell or tissue cultures. Various biomaterials and culture methods have been developed to modulate DO. Direct measurement of local DO in cultures has not been validated as a method to test DO modulation. In the present study we developed a DO measurement system equipped with a Clark-type oxygen microelectrode manipulated with 1 μm precision in three-dimensional space to explore potential applications for tissue engineering. By determining the microelectrode tip position precisely against the bottom plane of culture dishes with rat or human cardiac cells in static monolayer culture, we successfully obtained spatial distributions of DO in the medium. Theoretical quantitative predictions fit the obtained data well. Based on analyses of the variance between samples, we found the data reflected "local" oxygen consumption in the vicinity of the microelectrode and the detection of temporal changes in oxygen consumption rates of cultured cells was limited by the diffusion rate of oxygen in the medium. This oxygen measuring system monitors local oxygen consumption and production with high spatial resolution, and can potentially be used with recently developed oxygen modulating biomaterials to design microenvironments and non-invasively monitor local DO dynamics during culture.
|
| Volume |
112(6)
|
| Pages |
1263-74
|
| Published |
2015-6-1
|
| DOI |
10.1002/bit.25531
|
| PMID |
25565074
|
| MeSH |
Animals
Cell Culture Techniques / methods*
Culture Media / chemistry*
Humans
Microelectrodes
Myocytes, Cardiac / metabolism
Myocytes, Cardiac / physiology*
Oxygen / analysis*
Oxygen Consumption
Rats
|
| IF |
4.002
|
| Times Cited |
7
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
253G1(HPS0002) |
