RRC ID 36816
Author Nonomura K, Nakano M, Eiguchi M, Suzuki T, Kurata N.
Title PAIR2 is essential for homologous chromosome synapsis in rice meiosis I.
Journal J Cell Sci
Abstract The PAIR2 gene is required for homologous chromosome synapsis at meiosis I in rice (Oryza sativa L.) and encodes a HORMA-domain protein that is homologous to Saccharomyces cerevisiae HOP1 and Arabidopsis ASY1. Immunocytological and electron microscopic analyses indicate that PAIR2 proteins associate with axial elements (AEs) at leptotene and zygotene, and is removed from the AEs of arm regions when homologous chromosomes have been synapsed. Immunocytology against a centromeric histone H3 variant revealed that PAIR2 remains at centromeres until diakinesis, by which time the homologous centromeres had already been synapsed. However, neither precocious segregation of sister chromatids nor kinetochore dysfunction is observed, and AEs are normally assembled in the mutant. In the pair2-null mutant, homologous chromosome synapsis is completely eliminated. This study provides the first description of AE-associated protein in monocot plants and indicates that PAIR2 plays an essential role in promoting homologous chromosome synapsis. However, PAIR2 does not play a role in AE formation, sister chromatid cohesion at centromeres or kinetochore assembly in meiosis I of rice.
Volume 119(Pt 2)
Pages 217-25
Published 2006-1-15
DOI 10.1242/jcs.02736
PII 119/2/217
PMID 16410547
MeSH Cell Nucleus / metabolism Cell Nucleus / ultrastructure Chromosome Pairing* Chromosomes, Plant / metabolism Chromosomes, Plant / ultrastructure DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Genes, Plant* Meiosis / physiology* Oryza / cytology Oryza / genetics* Plant Proteins / genetics Plant Proteins / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism
IF 4.517
Times Cited 99
WOS Category CELL BIOLOGY
Resource
Rice Sterile strain (STER-NIG0007) (PAIR2)