論文 - 詳細
| RRC ID | 36823 |
|---|---|
| 著者 | Kato T, Yamashita H, Misawa T, Nishida K, Kurihara M, Tanaka M, Demizu Y, Oba M. |
| タイトル | Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids. |
| ジャーナル | Bioorg Med Chem |
| Abstract |
Cell-penetrating peptides (CPPs) have been developed as drug, protein, and gene delivery tools. In the present study, arginine (Arg)-rich CPPs containing unnatural amino acids were designed to deliver plasmid DNA (pDNA). The transfection ability of one of the Arg-rich CPPs examined here was more effective than that of the Arg nonapeptide, which is the most frequently used CPP. The transfection efficiencies of Arg-rich CPPs increased with longer post-incubation times and were significantly higher at 48-h and 72-h post-incubation than that of the commercially available transfection reagent TurboFect. These Arg-rich CPPs were complexed with pDNA for a long time in cells and effectively escaped from the late endosomes/lysosomes into the cytoplasm. These results will be helpful for designing novel CPPs for pDNA delivery. |
| 巻・号 | 24(12) |
| ページ | 2681-7 |
| 公開日 | 2016-6-15 |
| DOI | 10.1016/j.bmc.2016.04.031 |
| PII | S0968-0896(16)30275-9 |
| PMID | 27132868 |
| MeSH | Arginine / analogs & derivatives Arginine / metabolism* Cell Line Cell-Penetrating Peptides / chemistry Cell-Penetrating Peptides / metabolism* DNA / administration & dosage* DNA / genetics Humans Plasmids / administration & dosage* Plasmids / genetics Transfection / methods* |
| IF | 3.073 |
| 引用数 | 25 |
| WOS 分野 | CHEMISTRY, MEDICINAL CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAcc-Luc+ (RDB02443) |