RRC ID 36823
Author Kato T, Yamashita H, Misawa T, Nishida K, Kurihara M, Tanaka M, Demizu Y, Oba M.
Title Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids.
Journal Bioorg Med Chem
Abstract Cell-penetrating peptides (CPPs) have been developed as drug, protein, and gene delivery tools. In the present study, arginine (Arg)-rich CPPs containing unnatural amino acids were designed to deliver plasmid DNA (pDNA). The transfection ability of one of the Arg-rich CPPs examined here was more effective than that of the Arg nonapeptide, which is the most frequently used CPP. The transfection efficiencies of Arg-rich CPPs increased with longer post-incubation times and were significantly higher at 48-h and 72-h post-incubation than that of the commercially available transfection reagent TurboFect. These Arg-rich CPPs were complexed with pDNA for a long time in cells and effectively escaped from the late endosomes/lysosomes into the cytoplasm. These results will be helpful for designing novel CPPs for pDNA delivery.
Volume 24(12)
Pages 2681-7
Published 2016-6-15
DOI 10.1016/j.bmc.2016.04.031
PII S0968-0896(16)30275-9
PMID 27132868
MeSH Arginine / analogs & derivatives Arginine / metabolism* Cell Line Cell-Penetrating Peptides / chemistry Cell-Penetrating Peptides / metabolism* DNA / administration & dosage* DNA / genetics Humans Plasmids / administration & dosage* Plasmids / genetics Transfection / methods*
IF 3.073
Times Cited 25
DNA material pCAcc-Luc+ (RDB02443)