| RRC ID |
36823
|
| Author |
Kato T, Yamashita H, Misawa T, Nishida K, Kurihara M, Tanaka M, Demizu Y, Oba M.
|
| Title |
Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids.
|
| Journal |
Bioorg Med Chem
|
| Abstract |
Cell-penetrating peptides (CPPs) have been developed as drug, protein, and gene delivery tools. In the present study, arginine (Arg)-rich CPPs containing unnatural amino acids were designed to deliver plasmid DNA (pDNA). The transfection ability of one of the Arg-rich CPPs examined here was more effective than that of the Arg nonapeptide, which is the most frequently used CPP. The transfection efficiencies of Arg-rich CPPs increased with longer post-incubation times and were significantly higher at 48-h and 72-h post-incubation than that of the commercially available transfection reagent TurboFect. These Arg-rich CPPs were complexed with pDNA for a long time in cells and effectively escaped from the late endosomes/lysosomes into the cytoplasm. These results will be helpful for designing novel CPPs for pDNA delivery.
|
| Volume |
24(12)
|
| Pages |
2681-7
|
| Published |
2016-6-15
|
| DOI |
10.1016/j.bmc.2016.04.031
|
| PII |
S0968-0896(16)30275-9
|
| PMID |
27132868
|
| MeSH |
Arginine / analogs & derivatives
Arginine / metabolism*
Cell Line
Cell-Penetrating Peptides / chemistry
Cell-Penetrating Peptides / metabolism*
DNA / administration & dosage*
DNA / genetics
Humans
Plasmids / administration & dosage*
Plasmids / genetics
Transfection / methods*
|
| IF |
3.073
|
| Times Cited |
25
|
|
WOS Category
|
CHEMISTRY, MEDICINAL
CHEMISTRY, ORGANIC
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pCAcc-Luc+ (RDB02443) |
