RRC ID 36827
著者 Mora-Lorca JA, Sáenz-Narciso B, Gaffney CJ, Naranjo-Galindo FJ, Pedrajas JR, Guerrero-Gómez D, Dobrzynska A, Askjaer P, Szewczyk NJ, Cabello J, Miranda-Vizuete A.
タイトル Glutathione reductase gsr-1 is an essential gene required for Caenorhabditis elegans early embryonic development.
ジャーナル Free Radic Biol Med
Abstract Glutathione is the most abundant thiol in the vast majority of organisms and is maintained in its reduced form by the flavoenzyme glutathione reductase. In this work, we describe the genetic and functional analysis of the Caenorhabditis elegans gsr-1 gene that encodes the only glutathione reductase protein in this model organism. By using green fluorescent protein reporters we demonstrate that gsr-1 produces two GSR-1 isoforms, one located in the cytoplasm and one in the mitochondria. gsr-1 loss of function mutants display a fully penetrant embryonic lethal phenotype characterized by a progressive and robust cell division delay accompanied by an aberrant distribution of interphasic chromatin in the periphery of the cell nucleus. Maternally expressed GSR-1 is sufficient to support embryonic development but these animals are short-lived, sensitized to chemical stress, have increased mitochondrial fragmentation and lower mitochondrial DNA content. Furthermore, the embryonic lethality of gsr-1 worms is prevented by restoring GSR-1 activity in the cytoplasm but not in mitochondria. Given the fact that the thioredoxin redox systems are dispensable in C. elegans, our data support a prominent role of the glutathione reductase/glutathione pathway in maintaining redox homeostasis in the nematode.
巻・号 96
ページ 446-61
公開日 2016-7-1
DOI 10.1016/j.freeradbiomed.2016.04.017
PII S0891-5849(16)30035-1
PMID 27117030
PMC PMC8386055
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Cytoplasm / genetics Cytoplasm / metabolism Embryonic Development / genetics* Genes, Essential Glutathione / genetics Glutathione / metabolism* Glutathione Reductase / genetics* Glutathione Reductase / metabolism Mitochondria / genetics Mitochondria / metabolism Mutant Proteins / genetics Oxidation-Reduction Protein Isoforms / genetics Thioredoxins / genetics
IF 6.17
引用数 7
WOS 分野 ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 NA gsr-1(tm3574)