| RRC ID |
36858
|
| Author |
Li TC, Iwasaki K, Katano H, Kataoka M, Nagata N, Kobayashi K, Mizutani T, Takeda N, Wakita T, Suzuki T.
|
| Title |
Characterization of self-assembled virus-like particles of Merkel cell polyomavirus.
|
| Journal |
PLoS One
|
| Abstract |
In our recombinant baculovirus system, VP1 protein of merkel cell polyomavirus (MCPyV), which is implicated as a causative agent in Merkel cell carcinoma, was self-assembled into MCPyV-like particles (MCPyV-LP) with two different sizes in insect cells, followed by being released into the culture medium. DNA molecules of 1.5- to 5-kb, which were derived from host insect cells, were packaged in large, ~50-nm spherical particles but not in small, ~25-nm particles. Structure reconstruction using cryo-electron microscopy showed that large MCPyV-LPs are composed of 72 pentameric capsomeres arranged in a T = 7 icosahedral surface lattice and are 48 nm in diameter. The MCPyV-LPs did not share antigenic determinants with BK- and JC viruses (BKPyV and JCPyV). The VLP-based enzyme immunoassay was applied to investigate age-specific prevalence of MCPyV infection in the general Japanese population aged 1-70 years. While seroprevalence of MCPyV increased with age in children and young individuals, its seropositivity in each age group was lower compared with BKPyV and JCPyV.
|
| Volume |
10(2)
|
| Pages |
e0115646
|
| Published |
2015-1-1
|
| DOI |
10.1371/journal.pone.0115646
|
| PII |
PONE-D-14-39015
|
| PMID |
25671590
|
| PMC |
PMC4324643
|
| MeSH |
Adolescent
Adult
Aged
Animals
Baculoviridae / genetics
Capsid Proteins / genetics
Capsid Proteins / isolation & purification
Capsid Proteins / metabolism*
Cell Line
Child
Child, Preschool
Gene Expression
Humans
Infant
Insecta
JC Virus / immunology
Japan
Merkel cell polyomavirus* / genetics
Merkel cell polyomavirus* / metabolism
Middle Aged
Polyomavirus Infections / immunology
Young Adult
|
| IF |
2.74
|
| Times Cited |
8
|
|
WOS Category
|
VIROLOGY
|
| Resource |
| Human and Animal Cells |
Sf9(RCB0563) |
