Reference - Detail
| RRC ID | 36869 |
|---|---|
| Author | He J, Miyazaki J, Wang N, Tsurui H, Kobayashi T. |
| Title | Label-free imaging of melanoma with nonlinear photothermal microscopy. |
| Journal | Opt Lett |
| Abstract |
Nonlinear photothermal microscopy, in which the intensity of the pump heating beam is modulated at f and the photothermal signal is extracted from the probe beam with a lock-in amplifier referred to 2f, is applied to the imaging of mouse melanoma without any staining. The pump and probe pulses, with central wavelengths of 488 and 632 nm, and a pulse duration of ∼100 ps, are filtered from a compact commercial supercontinuum fiber laser source. An auto-balanced detector is applied to accumulate the signal and remove the laser noise of the probe. The spatial resolution of the nonlinear photothermal imaging is enhanced by ∼18% in both theoretical calculations and experiments, compared with a linear photothermal mechanism, and the resolution enhancement is theoretically ∼42% compared with conventional optical microscopy. This imaging technique shows possibilities for the clinical evaluation of melanoma with a high contrast and spatial resolution. |
| Volume | 40(7) |
| Pages | 1141-4 |
| Published | 2015-4-1 |
| DOI | 10.1364/OL.40.001141 |
| PII | 312932 |
| PMID | 25831277 |
| MeSH | Animals Hot Temperature* Melanoma / pathology* Mice Microscopy / methods* Molecular Imaging / methods* |
| IF | 3.714 |
| Times Cited | 12 |
| WOS Category | OPTICS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | B16 melanoma(RCB1283) |