RRC ID 3688
Author Matsuo Y, Asakawa K, Toda T, Katayama S.
Title A rapid method for protein extraction from fission yeast.
Journal Biosci Biotechnol Biochem
Abstract Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.
Volume 70(8)
Pages 1992-4
Published 2006-8-1
DOI 10.1271/bbb.60087
PII JST.JSTAGE/bbb/60087
PMID 16926515
MeSH Proteins / chemistry* Proteins / isolation & purification* Schizosaccharomyces / chemistry* Schizosaccharomyces / cytology Sodium Hydroxide / chemistry* Time Factors
IF 1.516
Times Cited 43
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast