RRC ID 36910
著者 Nakamura K, Yamashita K, Sawaki H, Waraya M, Katoh H, Nakayama N, Kawamata H, Nishimiya H, Ema A, Narimatsu H, Watanabe M.
タイトル Aberrant methylation of GCNT2 is tightly related to lymph node metastasis of primary CRC.
ジャーナル Anticancer Res
Abstract BACKGROUND:Glycoprotein expression profile is dramatically altered in human cancers; however, specific glycogenes have not been fully identified.
MATERIALS AND METHODS:A comprehensive real-time polymerase chain reaction (PCR) system for glycogenes (CRPS-G) identified several outstanding glycogenes. GCNT2 was of particular interest after GCNT2 expression and epigenetics were rigorously investigated in primary colorectal cancer (CRC).
RESULTS:The highlights of this work can be summarized as follows: (i) Expression of GCNT2 was remarkably suppressed. (ii) Silenced expression of GCNT2 was reactivated by combined demethylating agents. (iii) Promoter DNA methylation of GCNT2 was silenced in CRC cell lines and tissues. Hypomethylation of GCNT2 variant 2 is tightly associated with lymph node metastasis in primary CRC. (iv) GCNT2 methylation level in the normal tissues also showed a close association with that in the tumor tissues and reflected lymph node metastasis.
CONCLUSION:We identified aberrant expression of GCNT2, which can be explained by promoter DNA hypermethylation. Hypomethylation of the GCNT2 variant 2 reflected lymph node metastasis of CRC in the tumor and normal tissues.
巻・号 35(3)
ページ 1411-21
公開日 2015-3-1
PII 35/3/1411
PMID 25750292
MeSH Cell Line, Tumor Colorectal Neoplasms / genetics* Colorectal Neoplasms / pathology CpG Islands DNA Methylation* Humans Lymphatic Metastasis N-Acetylglucosaminyltransferases / genetics* Promoter Regions, Genetic
IF 1.994
引用数 13
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 CACO-2(RCB0988)