論文 - 詳細
| RRC ID | 36925 |
|---|---|
| 著者 | Terasawa K, Rajapakshe AR, Podyma-Inoue KA, Mishima-Tsumagari C, Yanagishita M, Hara-Yokoyama M. |
| タイトル | Preferential recognition of isocitrate dehydrogenase by a rabbit monoclonal antibody (ab124797) against the C-terminal peptide of RANKL. |
| ジャーナル | J Immunol Methods |
| Abstract |
A rabbit monoclonal antibody (Abcam ab124797), with high affinity for a synthetic peptide corresponding to the C-terminal region of the receptor activator of nuclear factor (NF)-κB ligand (RANKL), specifically recognizes a 37 kDa protein by immunoblotting, in good agreement with the molecular mass of RANKL. However, our mass spectroscopy analysis revealed that the protein recognized by the antibody is the α-subunit of NAD(+)-dependent isocitrate dehydrogenase (ICDH), a key Krebs cycle enzyme in mitochondria. Consistently, immunocytochemical staining with the antibody revealed a network organization characteristic of mitochondria, which overlapped with staining by MitoTracker and was lost after the siRNA-mediated downregulation of ICDH. The C-terminal peptide of ICDH contains similar chemical characteristics to that of the RANKL peptide and interacts with the antibody, although the affinity is a hundred times weaker. The present study provides an example of the preferential recognition of a surrogate protein by a rabbit monoclonal antibody. |
| 巻・号 | 420 |
| ページ | 1-10 |
| 公開日 | 2015-5-1 |
| DOI | 10.1016/j.jim.2015.03.006 |
| PII | S0022-1759(15)00070-8 |
| PMID | 25771969 |
| MeSH | Animals Antibodies, Monoclonal / immunology* Antibody Specificity* Cross Reactions Isocitrate Dehydrogenase / genetics Isocitrate Dehydrogenase / immunology* Mice Protein Structure, Tertiary RANK Ligand / genetics RANK Ligand / immunology* Rabbits |
| IF | 1.901 |
| 引用数 | 0 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) HeLa |