Reference - Detail
| RRC ID | 3693 |
|---|---|
| Author | Iwaki T, Iefuji H, Hiraga Y, Hosomi A, Morita T, Giga-Hama Y, Takegawa K. |
| Title | Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. |
| Journal | Microbiology (Reading) |
| Abstract |
Sterols are a major class of membrane lipids in eukaryotes. In Schizosaccharomyces pombe, sterol 24-C-methyltransferase (Erg6p), C-8 sterol isomerase (Erg2p), C-5 sterol desaturase (Erg31p, Erg32p), C-22 sterol desaturase (Erg5p) and C-24 (28) sterol reductase (Sts1p/Erg4p) have been predicted, but not yet determined, to catalyse a sequence of reactions from zymosterol to ergosterol. Disruption mutants of these genes were unable to synthesize ergosterol, and most were tolerant to the polyene drugs amphotericin B and nystatin. Disruption of erg31(+) or erg32(+) did not cause ergosterol deficiency or tolerance to polyene drugs, indicating that the two C-5 sterol desaturases have overlapping functions. GFP-tagged DRM (detergent-resistant membrane)-associated protein Pma1p localized to the plasma membrane in ergDelta mutants. DRM fractionation revealed that the association between Pma1-GFP and DRM was weakened in erg6Delta but not in other erg mutants. Several GFP-tagged plasma membrane proteins were tested, and an amino acid permease homologue, SPBC359.03c, was found to mislocalize to intracellular punctate structures in the ergDelta mutants. These results indicate that these proteins are responsible for ergosterol biosynthesis in fission yeast, similar to the situation in Saccharomyces cerevisiae. Furthermore, in fission yeast, ergosterol is important for plasma membrane structure and function and for localization of plasma membrane proteins. |
| Volume | 154(Pt 3) |
| Pages | 830-841 |
| Published | 2008-3-1 |
| DOI | 10.1099/mic.0.2007/011155-0 |
| PMID | 18310029 |
| MeSH | Amino Acid Transport Systems / analysis Amphotericin B / pharmacology Antifungal Agents / pharmacology Cell Fractionation Drug Resistance, Fungal / genetics Enzymes / genetics Enzymes / metabolism* Ergosterol / metabolism* Fungal Proteins / genetics Fungal Proteins / metabolism* Gene Deletion Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Membrane Proteins / analysis Mutagenesis, Insertional Nystatin / pharmacology Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Schizosaccharomyces / chemistry Schizosaccharomyces / drug effects Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics |
| IF | 2.138 |
| Times Cited | 47 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | FY15849(KJ100-7B) |