論文 - 詳細
RRC ID | 36956 |
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著者 | Shida-Sakazume T, Endo-Sakamoto Y, Unozawa M, Fukumoto C, Shimada K, Kasamatsu A, Ogawara K, Yokoe H, Shiiba M, Tanzawa H, Uzawa K. |
タイトル | Lysophosphatidylcholine acyltransferase1 overexpression promotes oral squamous cell carcinoma progression via enhanced biosynthesis of platelet-activating factor. |
ジャーナル | PLoS One |
Abstract |
BACKGROUND:The relevance of lysophosphatidylcholine acyltransferase1 (LPCAT1), a cytosolic enzyme in the remodeling pathway of phosphatidylcholine metabolism, in oral squamous cell carcinoma (OSCC) is unknown. We investigated LPCAT1 expression and its functional mechanism in OSCCs. METHODS:We analyzed LPCAT1 mRNA and protein expression levels in OSCC-derived cell lines. Immunohistochemistry was performed to identify correlations between LPCAT1 expression levels and primary OSCCs clinicopathological status. We established LPCAT1 knockdown models of the OSCC-derived cell lines (SAS, Ca9-22) for functional analysis and examined the association between LPCAT1 expression and the platelet-activating factor (PAF) concentration and PAF-receptor (PAFR) expression. RESULTS:LPCAT1 mRNA and protein were up-regulated significantly (p<0.05) in OSCC-derived cell lines compared with human normal oral keratinocytes. Immunohistochemistry showed significantly (p<0.05) elevated LPCAT1 expression in primary OSCCs compared with normal counterparts and a strong correlation between LPCAT1-positive OSCCs and tumoral size and regional lymph node metastasis. In LPCAT1 knockdown cells, cellular proliferation and invasiveness decreased significantly (p<0.05); cellular migration was inhibited compared with control cells. Down-regulation of LPCAT1 resulted in a decreased intercellular PAF concentration and PAFR expression. CONCLUSION:LPCAT1 was overexpressed in OSCCs and correlated with cellular invasiveness and migration. LPCAT1 may contribute to tumoral growth and metastasis in oral cancer. |
巻・号 | 10(3) |
ページ | e0120143 |
公開日 | 2015-1-1 |
DOI | 10.1371/journal.pone.0120143 |
PII | PONE-D-14-45533 |
PMID | 25803864 |
PMC | PMC4372572 |
MeSH | 1-Acylglycerophosphocholine O-Acyltransferase / genetics* 1-Acylglycerophosphocholine O-Acyltransferase / metabolism Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology Cell Line, Tumor Cell Proliferation Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Humans Mouth / metabolism Mouth / pathology* Mouth Neoplasms / genetics* Mouth Neoplasms / metabolism Mouth Neoplasms / pathology Neoplasm Invasiveness / genetics Neoplasm Invasiveness / pathology Platelet Activating Factor / metabolism* Platelet Membrane Glycoproteins / genetics Platelet Membrane Glycoproteins / metabolism RNA, Messenger / genetics Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism Tumor Cells, Cultured Up-Regulation* |
IF | 2.74 |
引用数 | 17 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | Sa3(RCB0980) HO-1-u-1(RCB2102) HSC-2(RCB1945) HSC-3(RCB1975) Ca9-22(RCB1976) |