論文 - 詳細
| RRC ID | 36966 |
|---|---|
| 著者 | Terao N, Takamatsu S, Minehira T, Sobajima T, Nakayama K, Kamada Y, Miyoshi E. |
| タイトル | Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes. |
| ジャーナル | World J Gastroenterol |
| Abstract |
AIM:To evaluate/isolate cancer stem cells (CSCs) from tissue or cell lines according to various definitions and cell surface markers. METHODS:Lectin microarray analysis was conducted on CSC-like fractions of the human pancreatic cancer cell line Panc1 by establishing anti-cancer drug-resistant cells. Changes in glycan structure of CSC-like cells were also investigated in sphere-forming cells as well as in CSC fractions obtained from overexpression of CD24 and CD44. RESULTS:Several types of fucosylation were increased under these conditions, and the expression of fucosylation regulatory genes such as fucosyltransferases, GDP-fucose synthetic enzymes, and GDP-fucose transporters were dramatically enhanced in CSC-like cells. These changes were significant in gemcitabine-resistant cells and sphere cells of a human pancreatic cancer cell line, Panc1. However, downregulation of cellular fucosylation by knockdown of the GDP-fucose transporter did not alter gemcitabine resistance, indicating that increased cellular fucosylation is a result of CSC-like transformation. CONCLUSION:Fucosylation might be a biomarker of CSC-like cells in pancreatic cancer. |
| 巻・号 | 21(13) |
| ページ | 3876-87 |
| 公開日 | 2015-4-7 |
| DOI | 10.3748/wjg.v21.i13.3876 |
| PMID | 25852272 |
| PMC | PMC4385534 |
| MeSH | Antimetabolites, Antineoplastic / pharmacology CD24 Antigen / genetics CD24 Antigen / metabolism Cell Line, Tumor Deoxycytidine / analogs & derivatives Deoxycytidine / pharmacology Dose-Response Relationship, Drug Drug Resistance, Neoplasm Fucose / metabolism* Fucosyltransferases / genetics Fucosyltransferases / metabolism Gemcitabine Glycosylation Humans Hyaluronan Receptors / genetics Hyaluronan Receptors / metabolism Neoplastic Stem Cells / drug effects Neoplastic Stem Cells / metabolism* Pancreatic Neoplasms / drug therapy Pancreatic Neoplasms / genetics Pancreatic Neoplasms / metabolism* RNA Interference Spheroids, Cellular Transfection |
| IF | 3.665 |
| 引用数 | 29 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | PK-59(RCB1901) |