Reference - Detail
| RRC ID | 36970 |
|---|---|
| Author | Arai Y, Hayakawa K, Arai D, Ito R, Iwasaki Y, Saito K, Akutsu K, Takatori S, Ishii R, Hayashi R, Izumi S, Sugino N, Kondo F, Horie M, Nakazawa H, Makino T, Hirosawa M, Shiota K, Ohgane J. |
| Title | Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells. |
| Journal | Biomed Res Int |
| Abstract |
The regulation of transcription and genome stability by epigenetic systems are crucial for the proper development of mammalian embryos. Chemicals that disturb epigenetic systems are termed epimutagens. We previously performed chemical screening that focused on heterochromatin formation and DNA methylation status in mouse embryonic stem cells and identified five epimutagens: diethyl phosphate (DEP), mercury (Hg), cotinine, selenium (Se), and octachlorodipropyl ether (S-421). Here, we used human induced pluripotent stem cells (hiPSCs) to confirm the effects of 20 chemicals, including the five epimutagens, detected at low concentrations in maternal peripheral and cord blood samples. Of note, these individual chemicals did not exhibit epimutagenic activity in hiPSCs. However, because the fetal environment contains various chemicals, we evaluated the effects of combined exposure to chemicals (DEP, Hg, cotinine, Se, and S-421) on hiPSCs. The combined exposure caused a decrease in the number of heterochromatin signals and aberrant DNA methylation status at multiple gene loci in hiPSCs. The combined exposure also affected embryoid body formation and neural differentiation from hiPSCs. Therefore, DEP, Hg, cotinine, Se, and S-421 were defined as an "epimutagen combination" that is effective at low concentrations as detected in maternal peripheral and cord blood. |
| Volume | 2015 |
| Pages | 876047 |
| Published | 2015-1-1 |
| DOI | 10.1155/2015/876047 |
| PMID | 26339649 |
| PMC | PMC4538592 |
| MeSH | Animals Cell Differentiation / drug effects Cotinine / toxicity DNA Methylation / drug effects* Embryonic Development / drug effects Epigenesis, Genetic / drug effects* Epigenesis, Genetic / genetics Ethers / toxicity Female Fetal Blood / drug effects Heterochromatin / drug effects Heterochromatin / genetics Humans Induced Pluripotent Stem Cells / drug effects* Mercury / toxicity Mice Mutagens / toxicity* Organophosphates / toxicity Selenium / toxicity |
| IF | 2.276 |
| Times Cited | 3 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 201B7(HPS0063) MC3T3-G2/PA6(RCB1127) |