RRC ID 36979
Author Yazaki Y, Oyane A, Sogo Y, Ito A, Yamazaki A, Tsurushima H.
Title Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers.
Journal Int J Mol Sci
Abstract Surface-mediated gene transfer systems using biocompatible calcium phosphate (CaP)-based composite layers have attracted attention as a tool for controlling cell behaviors. In the present study we aimed to demonstrate the potential of CaP-based composite layers to mediate area-specific dual gene transfer and to stimulate cells on an area-by-area basis in the same well. For this purpose we prepared two pairs of DNA-fibronectin-apatite composite (DF-Ap) layers using a pair of reporter genes and pair of differentiation factor genes. The results of the area-specific dual gene transfer successfully demonstrated that the cells cultured on a pair of DF-Ap layers that were adjacently placed in the same well showed specific gene expression patterns depending on the gene that was immobilized in the underlying layer. Moreover, preliminary real-time PCR results indicated that multipotential C3H10T1/2 cells may have a potential to change into different types of cells depending on the differentiation factor gene that was immobilized in the underlying layer, even in the same well. Because DF-Ap layers have a potential to mediate area-specific cell stimulation on their surfaces, they could be useful in tissue engineering applications.
Volume 16(4)
Pages 8294-309
Published 2015-4-14
DOI 10.3390/ijms16048294
PII ijms16048294
PMID 25874757
PMC PMC4425081
MeSH Animals Apatites / chemistry* CHO Cells Cell Differentiation Cricetinae Cricetulus DNA / chemistry Fibronectins / chemistry Genes, Reporter Luciferases, Firefly / biosynthesis Luciferases, Firefly / genetics Luciferases, Renilla / biosynthesis Luciferases, Renilla / genetics Mice Surface Properties Transfection / methods*
IF 3.687
Times Cited 0
Human and Animal Cells