| RRC ID |
36979
|
| 著者 |
Yazaki Y, Oyane A, Sogo Y, Ito A, Yamazaki A, Tsurushima H.
|
| タイトル |
Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Surface-mediated gene transfer systems using biocompatible calcium phosphate (CaP)-based composite layers have attracted attention as a tool for controlling cell behaviors. In the present study we aimed to demonstrate the potential of CaP-based composite layers to mediate area-specific dual gene transfer and to stimulate cells on an area-by-area basis in the same well. For this purpose we prepared two pairs of DNA-fibronectin-apatite composite (DF-Ap) layers using a pair of reporter genes and pair of differentiation factor genes. The results of the area-specific dual gene transfer successfully demonstrated that the cells cultured on a pair of DF-Ap layers that were adjacently placed in the same well showed specific gene expression patterns depending on the gene that was immobilized in the underlying layer. Moreover, preliminary real-time PCR results indicated that multipotential C3H10T1/2 cells may have a potential to change into different types of cells depending on the differentiation factor gene that was immobilized in the underlying layer, even in the same well. Because DF-Ap layers have a potential to mediate area-specific cell stimulation on their surfaces, they could be useful in tissue engineering applications.
|
| 巻・号 |
16(4)
|
| ページ |
8294-309
|
| 公開日 |
2015-4-14
|
| DOI |
10.3390/ijms16048294
|
| PII |
ijms16048294
|
| PMID |
25874757
|
| PMC |
PMC4425081
|
| MeSH |
Animals
Apatites / chemistry*
CHO Cells
Cell Differentiation
Cricetinae
Cricetulus
DNA / chemistry
Fibronectins / chemistry
Genes, Reporter
Luciferases, Firefly / biosynthesis
Luciferases, Firefly / genetics
Luciferases, Renilla / biosynthesis
Luciferases, Renilla / genetics
Mice
Surface Properties
Transfection / methods*
|
| IF |
4.556
|
| 引用数 |
0
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1(RCB0285)
10T1/2(RCB0247) |
