RRC ID |
36983
|
著者 |
Kaminaga K, Noguchi M, Narita A, Sakamoto Y, Kanari Y, Yokoya A.
|
タイトル |
Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators.
|
ジャーナル |
Radiat Prot Dosimetry
|
Abstract |
To explore the effects of X-ray irradiation on mammalian cell cycle dynamics, single cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci) technique were tracked. HeLa cells expressing Fucci were used to visualise cell cycle modifications induced by irradiation. After cultured HeLa-Fucci cells were exposed to 5 Gy X-rays, fluorescent cell images were captured every 20 min for 48 h using a fluorescent microscope. Time dependence of the fluorescence intensity of S/G2 cells was analysed to examine the cell cycle dynamics of irradiated and non-irradiated control cells. The results showed that irradiated cells could be divided into two populations: one with similar cell cycle dynamics to that of non-irradiated cells, and another displaying a prolonged G2 phase. Based on these findings, it is proposed in this article that an underlying switch mechanism is involved in cell cycle regulation and the G2/M checkpoint of HeLa cells.
|
巻・号 |
166(1-4)
|
ページ |
91-4
|
公開日 |
2015-9-1
|
DOI |
10.1093/rpd/ncv168
|
PII |
ncv168
|
PMID |
25877544
|
MeSH |
Fluorescence
Fluorescent Dyes / analysis*
G2 Phase Cell Cycle Checkpoints / radiation effects*
Gamma Rays / adverse effects*
HeLa Cells
Humans
Indicators and Reagents
Microscopy, Fluorescence
S Phase Cell Cycle Checkpoints / radiation effects*
Ubiquitination / radiation effects*
X-Rays
|
IF |
0.773
|
引用数 |
5
|
WOS 分野
|
NUCLEAR SCIENCE & TECHNOLOGY
PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
ENVIRONMENTAL SCIENCES
|
リソース情報 |
ヒト・動物細胞 |
HeLa/Fucci(RCB2812) |