| RRC ID |
36993
|
| Author |
Yokoo T, Matsumoto K, Ooba T, Morimoto K, Taguchi S.
|
| Title |
Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells.
|
| Volume |
79(6)
|
| Pages |
986-8
|
| Published |
2015-1-1
|
| DOI |
10.1080/09168451.2014.1002448
|
| PMID |
25647430
|
| MeSH |
Alcohol Oxidoreductases / genetics*
Alcohol Oxidoreductases / metabolism
Biocatalysis
Cell Line
Hydroxybutyrates / metabolism*
Kinetics
Plants, Genetically Modified
Polyesters / metabolism*
Protein Engineering*
Tobacco / cytology*
Tobacco / genetics*
Transformation, Genetic
|
| IF |
1.516
|
| Times Cited |
3
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, APPLIED
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00001 |
