Reference - Detail
| RRC ID | 37020 |
|---|---|
| Author | Ebina H, Kanemura Y, Misawa N, Sakuma T, Kobayashi T, Yamamoto T, Koyanagi Y. |
| Title | A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector. |
| Journal | PLoS One |
| Abstract |
DNA-editing technology has made it possible to rewrite genetic information in living cells. Human immunodeficiency virus (HIV) provirus, an integrated form of viral complementary DNA in host chromosomes, could be a potential target for this technology. We recently reported that HIV proviral DNA could be excised from the chromosomal DNA of HIV-based lentiviral DNA-transduced T cells after multiple introductions of a clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 endonuclease system targeting HIV long terminal repeats (LTR). Here, we generated a more efficient strategy that enables the excision of HIV proviral DNA using customized transcription activator-like effector nucleases (TALENs) targeting the same HIV LTR site. A single transfection of TALEN-encoding mRNA, prepared from in vitro transcription, resulted in more than 80% of lentiviral vector DNA being successfully removed from the T cell lines. Furthermore, we developed a lentiviral vector system that takes advantage of the efficient proviral excision with TALENs and permits the simple selection of gene-transduced and excised cells in T cell lines. |
| Volume | 10(3) |
| Pages | e0120047 |
| Published | 2015-1-1 |
| DOI | 10.1371/journal.pone.0120047 |
| PII | PONE-D-14-49017 |
| PMID | 25781496 |
| PMC | PMC4363575 |
| MeSH | CRISPR-Cas Systems / genetics* Cell Line DNA, Viral / genetics* DNA, Viral / metabolism Genetic Vectors / genetics* Genetic Vectors / metabolism HIV-1 / genetics* HIV-1 / metabolism Humans Transduction, Genetic* Virus Integration / genetics* |
| IF | 2.74 |
| Times Cited | 39 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) Jurkat(RCB0806) |