Reference - Detail
| RRC ID | 37040 |
|---|---|
| Author | Kato S, Masuda Y, Konishi M, Oikawa T. |
| Title | Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography. |
| Journal | J Pharm Biomed Anal |
| Abstract |
The intrinsic D-amino acid profile of mouse macrophages extracted from the peritoneal cavity was analyzed using high performance liquid chromatography. Six D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu, D-Gln and D-Lys) were detected in cell lysates of mouse macrophages. The content and the D/D+L ratio differed depending on the type of D-amino acid and were approximately 3.5-22 nmol/g cells, and approximately 1-20%, respectively. The D-amino acid composition of RAW 264.7 cells, which is a model macrophage cell line, was similar to that of the mouse macrophage. These results suggest that macrophages and RAW 264.7 cells with macrophage-like functions have a similar D-amino acid profile. |
| Volume | 116 |
| Pages | 101-4 |
| Published | 2015-12-10 |
| DOI | 10.1016/j.jpba.2015.04.028 |
| PII | S0731-7085(15)00261-7 |
| PMID | 25982753 |
| MeSH | Amino Acids / analysis* Amino Acids / chemistry* Amino Acids / metabolism Animals Cell Line Chromatography, High Pressure Liquid / methods Macrophages / chemistry* Macrophages / metabolism Mice Mice, Inbred C57BL Stereoisomerism |
| IF | 3.209 |
| Times Cited | 6 |
| WOS Category | PHARMACOLOGY & PHARMACY CHEMISTRY, ANALYTICAL |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |