論文 - 詳細
| RRC ID | 37066 |
|---|---|
| 著者 | Hosokawa-Muto J, Fujinami Y, Mizuno N. |
| タイトル | Evaluation of the Universal Viral Transport system for long-term storage of virus specimens for microbial forensics. |
| ジャーナル | J Forensic Leg Med |
| Abstract |
Forensic microbial specimens, including bacteria and viruses, are collected at biocrime and bioterrorism scenes. Although it is preferable that the pathogens in these samples are alive and kept in a steady state, the samples may be stored for prolonged periods before analysis. Therefore, it is important to understand the effects of storage conditions on the pathogens contained within such samples. To evaluate the capacity to preserve viable virus and the viral genome, influenza virus was added to the transport medium of the Universal Viral Transport system and stored for over 3 months at various temperatures, after which virus titrations and quantitative analysis of the influenza hemagglutinin gene were performed. Although viable viruses became undetectable 29 days after the medium was stored at room temperature, viruses in the medium stored at 4°C were viable even after 99 days. A quantitative PCR analysis indicated that the hemagglutinin gene was maintained for 99 days at both 4°C and room temperature. Therefore, long-term storage at 4°C has little effect on viable virus and viral genes, so the Universal Viral Transport system can be useful for microbial forensics. This study provides important information for the handling of forensic virus specimens. |
| 巻・号 | 34 |
| ページ | 29-33 |
| 公開日 | 2015-8-1 |
| DOI | 10.1016/j.jflm.2015.04.019 |
| PII | S1752-928X(15)00082-7 |
| PMID | 26165655 |
| MeSH | Hemagglutinins, Viral / genetics Humans Microbial Viability Orthomyxoviridae / isolation & purification* Polymerase Chain Reaction RNA, Viral Real-Time Polymerase Chain Reaction Specimen Handling / methods* Temperature |
| IF | 1.302 |
| 引用数 | 0 |
| WOS 分野 | MEDICINE, LEGAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |