Reference - Detail
| RRC ID | 37074 |
|---|---|
| Author | Saeki N, Ono H, Sakamoto H, Yoshida T. |
| Title | Down-regulation of Immune-related Genes by PSCA in Gallbladder Cancer Cells Implanted into Mice. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:In previous work, we found that prostate stem cell antigen (PSCA) gene, encoding a glycosylphosphatidylinositol-anchored protein, is a presumable tumor suppressor in gastric cancer and gallbladder cancer (GBC). The introduction of PSCA cDNA into GBC cell lines significantly suppressed tumorigenecity of cells in mice. The PSCA protein is thought to be involved in some form of intracellular signaling that remains to be elucidated. MATERIALS AND METHODS:Using microarrays, we conducted gene-expression profiling on tumors generated by a GBC cell line TGBC-1TKB, with and without expression of PSCA, which was implanted into mice. Genes whose expression was down-regulated by PSCA were selected, and their down-regulation was confirmed by real-time PCR. RESULTS:We identified several immune-related genes down-regulated by PSCA, including interleukin 8 (IL8), IL1 receptor antagonist (IL1RN) and S100 calcium-binding proteins A8 (S100A8) and A9 (S100A9). CONCLUSION:PSCA signaling may suppress tumor growth in vivo by modulating immunological characteristics of GBC cells. |
| Volume | 35(5) |
| Pages | 2619-25 |
| Published | 2015-5-1 |
| PII | 35/5/2619 |
| PMID | 25964537 |
| MeSH | Animals Antigens, Neoplasm / biosynthesis Antigens, Neoplasm / genetics* Antigens, Neoplasm / immunology Calgranulin A / biosynthesis Calgranulin A / immunology Calgranulin B / biosynthesis Calgranulin B / immunology Cell Line, Tumor GPI-Linked Proteins / biosynthesis GPI-Linked Proteins / genetics GPI-Linked Proteins / immunology Gallbladder Neoplasms / genetics* Gallbladder Neoplasms / immunology Gallbladder Neoplasms / pathology Gene Expression Regulation, Neoplastic Glycosylphosphatidylinositols / biosynthesis* Glycosylphosphatidylinositols / genetics Glycosylphosphatidylinositols / immunology Humans Interleukin 1 Receptor Antagonist Protein / biosynthesis Interleukin 1 Receptor Antagonist Protein / immunology Interleukin-8 / biosynthesis Interleukin-8 / immunology Mice Neoplasm Proteins / biosynthesis Neoplasm Proteins / genetics* Neoplasm Proteins / immunology |
| IF | 1.994 |
| Times Cited | 7 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | TGBC1TKB(RCB1129) G-415(RCB2640) |