| RRC ID |
37087
|
| 著者 |
Tazawa H, Sato K, Tsutiya A, Tokeshi M, Ohtani-Kaneko R.
|
| タイトル |
A microfluidic cell culture system for monitoring of sequential changes in endothelial cells after heat stress.
|
| ジャーナル |
Thromb Res
|
| Abstract |
Endothelial damage induced by a highly elevated body temperature is crucial in some diseases including viral hemorrhagic fevers. Here, we report the heat-induced sequential changes of endothelial cells under shear stress, which were determined with a microfluidic culture system. Although live cell imaging showed only minor changes in the appearance of heat-treated cells, Hsp70 mRNA expression analysis demonstrated that the endothelial cells in channels of the system responded well to heat treatment. F-actin staining also revealed clear changes in the bundles of actin filaments after heat treatment. Well-organized bundles of actin filaments in control cells disappeared in heat-treated cells cultured in the channel. Furthermore, the system enabled detection of sequential changes in plasminogen activator inhibitor-1 (PAI-1) secretion from endothelial cells. PAI-1 concentration in the effluent solution was significantly elevated for the first 15min after initiation of heat treatment, and then decreased subsequently. This study provides fundamental information on heat-induced endothelial changes under shear stress and introduces a potent tool for analyzing endothelial secretions.
|
| 巻・号 |
136(2)
|
| ページ |
328-34
|
| 公開日 |
2015-8-1
|
| DOI |
10.1016/j.thromres.2015.05.008
|
| PII |
S0049-3848(15)00235-2
|
| PMID |
26044666
|
| MeSH |
Actin Cytoskeleton / metabolism
Adaptation, Physiological / physiology
Animals
Cell Line
Cell Separation / instrumentation*
Endothelial Cells / cytology
Endothelial Cells / physiology*
Equipment Design
Equipment Failure Analysis
HSP70 Heat-Shock Proteins / metabolism*
Haplorhini
Heat-Shock Response / physiology*
Heating / instrumentation*
Hot Temperature
Humans
Lab-On-A-Chip Devices*
Plasminogen Activator Inhibitor 1 / metabolism
|
| IF |
2.869
|
| 引用数 |
9
|
|
WOS 分野
|
PERIPHERAL VASCULAR DISEASE
HEMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RF/6A 135(RCB1556) |
