RRC ID 37087
Author Tazawa H, Sato K, Tsutiya A, Tokeshi M, Ohtani-Kaneko R.
Title A microfluidic cell culture system for monitoring of sequential changes in endothelial cells after heat stress.
Journal Thromb Res
Abstract Endothelial damage induced by a highly elevated body temperature is crucial in some diseases including viral hemorrhagic fevers. Here, we report the heat-induced sequential changes of endothelial cells under shear stress, which were determined with a microfluidic culture system. Although live cell imaging showed only minor changes in the appearance of heat-treated cells, Hsp70 mRNA expression analysis demonstrated that the endothelial cells in channels of the system responded well to heat treatment. F-actin staining also revealed clear changes in the bundles of actin filaments after heat treatment. Well-organized bundles of actin filaments in control cells disappeared in heat-treated cells cultured in the channel. Furthermore, the system enabled detection of sequential changes in plasminogen activator inhibitor-1 (PAI-1) secretion from endothelial cells. PAI-1 concentration in the effluent solution was significantly elevated for the first 15min after initiation of heat treatment, and then decreased subsequently. This study provides fundamental information on heat-induced endothelial changes under shear stress and introduces a potent tool for analyzing endothelial secretions.
Volume 136(2)
Pages 328-34
Published 2015-8-1
DOI 10.1016/j.thromres.2015.05.008
PII S0049-3848(15)00235-2
PMID 26044666
MeSH Actin Cytoskeleton / metabolism Adaptation, Physiological / physiology Animals Cell Line Cell Separation / instrumentation* Endothelial Cells / cytology Endothelial Cells / physiology* Equipment Design Equipment Failure Analysis HSP70 Heat-Shock Proteins / metabolism* Haplorhini Heat-Shock Response / physiology* Heating / instrumentation* Hot Temperature Humans Lab-On-A-Chip Devices* Plasminogen Activator Inhibitor 1 / metabolism
IF 2.869
Times Cited 9
Human and Animal Cells RF/6A 135(RCB1556)