論文 - 詳細
| RRC ID | 37088 |
|---|---|
| 著者 | Kim BJ, Choi YS, Choi BH, Lim S, Song YH, Cha HJ. |
| タイトル | Mussel adhesive protein fused with cell adhesion recognition motif triggers integrin-mediated adhesion and signaling for enhanced cell spreading, proliferation, and survival. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
Adhesion of cells to a surface is a basic and important requirement in the fields of cell culture and tissue engineering. Previously, we constructed the cell adhesive, fp-151-RGD, by fusion of the hybrid mussel adhesive protein, fp-151, and GRGDSP peptide, one of the major cell adhesion recognition motifs; fp-151-RGD efficiently immobilized cells on coated culture surfaces with no protein and surface modifications, and apparently enhanced cell adhesion, proliferation, and spreading abilities. In the present study, we investigated the potential use of fp-151-RGD as a biomimetic extracellular matrix material at the molecular level by elucidating its substantial effects on integrin-mediated adhesion and signaling. Apoptosis derived from serum deprivation was significantly suppressed on the fp-151-RGD-coated surface, indicating that RGD-induced activation of integrin-mediated signaling triggers the pathway for cell survival. Analysis of the phosphorylation of focal adhesion kinase clearly demonstrated activation of focal adhesion kinase, a well-established indicator of integrin-mediated signaling, on the fp-151-RGD-coated surface, leading to significantly enhanced cell behaviors, including proliferation, spreading and survival, and consequently, more efficient cell culture. |
| 巻・号 | 94(3) |
| ページ | 886-92 |
| 公開日 | 2010-9-1 |
| DOI | 10.1002/jbm.a.32768 |
| PMID | 20336754 |
| MeSH | Amino Acid Motifs* Animals Apoptosis Cell Adhesion / physiology* Cell Proliferation* Cell Survival / physiology* Focal Adhesion Protein-Tyrosine Kinases / metabolism Mice NIH 3T3 Cells Oligopeptides / genetics Oligopeptides / metabolism Proteins / genetics Proteins / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction / physiology* |
| IF | 3.525 |
| 引用数 | 13 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |