RRC ID 37103
Author Nagayama K, Hamaji Y, Sato Y, Matsumoto T.
Title Mechanical trapping of the nucleus on micropillared surfaces inhibits the proliferation of vascular smooth muscle cells but not cervical cancer HeLa cells.
Journal J Biomech
Abstract The interaction between cells and the extracellular matrix on a topographically patterned surface can result in changes in cell shape and many cellular functions. In the present study, we demonstrated the mechanical deformation and trapping of the intracellular nucleus using polydimethylsiloxane (PDMS)-based microfabricated substrates with an array of micropillars. We investigated the differential effects of nuclear deformation on the proliferation of healthy vascular smooth muscle cells (SMCs) and cervical cancer HeLa cells. Both types of cell spread normally in the space between micropillars and completely invaded the extracellular microstructures, including parts of their cytoplasm and their nuclei. We found that the proliferation of SMCs but not HeLa cells was dramatically inhibited by cultivation on the micropillar substrates, even though remarkable deformation of nuclei was observed in both types of cells. Mechanical testing with an atomic force microscope and a detailed image analysis with confocal microscopy revealed that SMC nuclei had a thicker nuclear lamina and greater expression of lamin A/C than those of HeLa cells, which consequently increased the elastic modulus of the SMC nuclei and their nuclear mechanical resistance against extracellular microstructures. These results indicate that the inhibition of cell proliferation resulted from deformation of the mature lamin structures, which might be exposed to higher internal stress during nuclear deformation. This nuclear stress-induced inhibition of cell proliferation occurred rarely in cancer cells with deformable nuclei.
Volume 48(10)
Pages 1796-803
Published 2015-7-16
DOI 10.1016/j.jbiomech.2015.05.004
PII S0021-9290(15)00281-X
PMID 26054426
MeSH Cell Nucleus / drug effects* Cell Nucleus / metabolism Cell Proliferation / drug effects Cytoplasm / drug effects Dimethylpolysiloxanes / pharmacology Elastic Modulus Extracellular Matrix / drug effects Female HeLa Cells Humans Mechanical Phenomena* Microtechnology / instrumentation* Muscle, Smooth, Vascular / cytology* Muscle, Smooth, Vascular / drug effects* Uterine Cervical Neoplasms / pathology*
IF 2.32
Times Cited 21
Human and Animal Cells HeLa(RCB0007)