RRC ID 37120
Author Ueno Y, Yoshida R, Kishi-Kaboshi M, Matsushita A, Jiang CJ, Goto S, Takahashi A, Hirochika H, Takatsuji H.
Title MAP kinases phosphorylate rice WRKY45.
Journal Plant Signal Behav
Abstract WRKY45 transcription factor is a central regulator of disease resistance mediated by the salicylic acid (SA) signaling pathway in rice. SA-activated WRKY45 protein induces the accumulation of its own mRNA. However, the mechanism underlying this regulation is still unknown. Here, we report three lines of evidence showing that a mitogen-activated protein kinase (MAPK) cascade is involved in this regulation. An inhibitor of MAPK kinase (MAPKK) suppressed the increase in WRKY45 transcript level in response to SA. Two MAPKs, OsMPK4 and OsMPK6, phosphorylated WRKY45 protein in vitro. The activity of OsMPK6 was rapidly upregulated by SA treatment in rice cells. These results suggest that WRKY45 is regulated by MAPK-dependent phosphorylation in the SA pathway.
Volume 8(6)
Pages e24510
Published 2013-6-1
DOI 10.4161/psb.24510
PII 24510
PMID 23603961
PMC PMC3908937
MeSH Mitogen-Activated Protein Kinases / metabolism* Oryza / enzymology* Phosphorylation Plant Proteins / metabolism* Salicylic Acid / metabolism Signal Transduction
IF 1.644
Times Cited 18
Resource
Arabidopsis / Cultured plant cells, genes rpc00031