Reference - Detail
RRC ID | 37142 |
---|---|
Author | Ota S, Oshima K, Yamazaki T, Kim S, Yu Z, Yoshihara M, Takeda K, Takeshita T, Hirata A, Bišová K, Zachleder V, Hattori M, Kawano S. |
Title | Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. |
Journal | Biotechnol Biofuels |
Abstract |
BACKGROUND:Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and for biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, and starch productivity levels. However, further biotechnological exploitation has been hampered by a lack of genomic information. RESULTS:Here, we sequenced the whole genome and transcriptome, and analyzed the behavior of P. kessleri NIES-2152 under lipid production-inducing conditions. The assembly includes 13,057 protein-coding genes in a 62.5-Mbp nuclear genome. Under conditions of sulfur deprivation, lipid accumulation was correlated with the transcriptomic induction of enzymes involved in sulfur metabolism, triacylglycerol (TAG) synthesis, autophagy, and remodeling of light-harvesting complexes. CONCLUSIONS:Three-dimensional transmission electron microscopy (3D-TEM) revealed extensive alterations in cellular anatomy accompanying lipid hyperaccumulation. The present 3D-TEM results, together with transcriptomic data support the finding that upregulation of TAG synthesis and autophagy are potential key mediators of the hyperaccumulation of lipids under conditions of nutrient stress. |
Volume | 9 |
Pages | 13 |
Published | 2016-1-1 |
DOI | 10.1186/s13068-016-0424-2 |
PII | 424 |
PMID | 26811738 |
PMC | PMC4724957 |
IF | 4.815 |
Times Cited | 35 |
WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENERGY & FUELS |
Resource | |
Algae | NIES-2152 |