| RRC ID |
37155
|
| 著者 |
Wen Z, Liu Z, Hou Y, Liu C, Gao F, Zheng Y, Chen F.
|
| タイトル |
Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis.
|
| ジャーナル |
Enzyme Microb Technol
|
| Abstract |
Haematococcus pluvialis is one of the most promising natural sources of astaxanthin. However, inducing the accumulation process has become one of the primary obstacles in astaxanthin production. In this study, the effect of ethanol on astaxanthin accumulation was investigated. The results demonstrated that astaxanthin accumulation occurred with ethanol addition even under low-light conditions. The astaxanthin productivity could reach 11.26 mg L(-1) d(-1) at 3% (v/v) ethanol, which was 2.03 times of that of the control. The transcriptional expression patterns of eight carotenogenic genes were evaluated using real-time PCR. The results showed that ethanol greatly enhanced transcription of the isopentenyl diphosphate (IPP) isomerase genes (ipi-1 and ipi-2), which were responsible for isomerization reaction of IPP and dimethylallyl diphosphate (DMAPP). This finding suggests that ethanol induced astaxanthin biosynthesis was up-regulated mainly by ipi-1 and ipi-2 at transcriptional level, promoting isoprenoid synthesis and substrate supply to carotenoid formation. Thus ethanol has the potential to be used as an effective reagent to induce astaxanthin accumulation in H. pluvialis.
|
| 巻・号 |
78
|
| ページ |
10-7
|
| 公開日 |
2015-10-1
|
| DOI |
10.1016/j.enzmictec.2015.06.010
|
| PII |
S0141-0229(15)30016-8
|
| PMID |
26215339
|
| MeSH |
Algal Proteins / genetics
Algal Proteins / metabolism
Antioxidants / metabolism
Carbon-Carbon Double Bond Isomerases / genetics
Carbon-Carbon Double Bond Isomerases / metabolism
Ethanol / metabolism
Ethanol / pharmacology*
Gene Expression / drug effects
Gene Expression / radiation effects
Hemiterpenes / metabolism
Light
Microscopy, Electron, Scanning
Organophosphorus Compounds / metabolism
Real-Time Polymerase Chain Reaction
Substrate Specificity
Volvocida / drug effects*
Volvocida / genetics
Volvocida / metabolism*
Xanthophylls / biosynthesis
Xanthophylls / metabolism
|
| IF |
3.448
|
| 引用数 |
23
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-144 |
