論文 - 詳細
| RRC ID | 37155 |
|---|---|
| 著者 | Wen Z, Liu Z, Hou Y, Liu C, Gao F, Zheng Y, Chen F. |
| タイトル | Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis. |
| ジャーナル | Enzyme Microb Technol |
| Abstract |
Haematococcus pluvialis is one of the most promising natural sources of astaxanthin. However, inducing the accumulation process has become one of the primary obstacles in astaxanthin production. In this study, the effect of ethanol on astaxanthin accumulation was investigated. The results demonstrated that astaxanthin accumulation occurred with ethanol addition even under low-light conditions. The astaxanthin productivity could reach 11.26 mg L(-1) d(-1) at 3% (v/v) ethanol, which was 2.03 times of that of the control. The transcriptional expression patterns of eight carotenogenic genes were evaluated using real-time PCR. The results showed that ethanol greatly enhanced transcription of the isopentenyl diphosphate (IPP) isomerase genes (ipi-1 and ipi-2), which were responsible for isomerization reaction of IPP and dimethylallyl diphosphate (DMAPP). This finding suggests that ethanol induced astaxanthin biosynthesis was up-regulated mainly by ipi-1 and ipi-2 at transcriptional level, promoting isoprenoid synthesis and substrate supply to carotenoid formation. Thus ethanol has the potential to be used as an effective reagent to induce astaxanthin accumulation in H. pluvialis. |
| 巻・号 | 78 |
| ページ | 10-7 |
| 公開日 | 2015-10-1 |
| DOI | 10.1016/j.enzmictec.2015.06.010 |
| PII | S0141-0229(15)30016-8 |
| PMID | 26215339 |
| MeSH | Algal Proteins / genetics Algal Proteins / metabolism Antioxidants / metabolism Carbon-Carbon Double Bond Isomerases / genetics Carbon-Carbon Double Bond Isomerases / metabolism Ethanol / metabolism Ethanol / pharmacology* Gene Expression / drug effects Gene Expression / radiation effects Hemiterpenes / metabolism Light Microscopy, Electron, Scanning Organophosphorus Compounds / metabolism Real-Time Polymerase Chain Reaction Substrate Specificity Volvocida / drug effects* Volvocida / genetics Volvocida / metabolism* Xanthophylls / biosynthesis Xanthophylls / metabolism |
| IF | 3.448 |
| 引用数 | 23 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-144 |