| RRC ID |
37460
|
| Author |
Shiraishi H.
|
| Title |
Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately -1 °C min(-1) in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately -1 °C min(-1) in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains.
|
| Volume |
80(10)
|
| Pages |
2051-7
|
| Published |
2016-10-1
|
| DOI |
10.1080/09168451.2016.1189320
|
| PMID |
27240586
|
| MeSH |
Cell Proliferation
Cell Survival
Cryopreservation / methods*
Species Specificity
Spirulina / cytology*
|
| IF |
1.516
|
| Times Cited |
5
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
|
| Resource |
| Algae |
NIES-39
NIES-46
NIES-2308 |
