RRC ID 37464
Author Sun LW, Jiang WJ, Sato H, Kawachi M, Lu XW.
Title Rapid Classification and Identification of Microcystis aeruginosa Strains Using MALDI-TOF MS and Polygenetic Analysis.
Journal PLoS One
Abstract Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was used to establish a rapid, simple, and accurate method to differentiate among strains of Microcystis aeruginosa, one of the most prevalent types of bloom-forming cyanobacteria. M. aeruginosa NIES-843, for which a complete genome has been sequenced, was used to characterize ribosomal proteins as biomarkers and to optimize conditions for observing ribosomal proteins as major peaks in a given mass spectrum. Thirty-one of 52 ribosomal subunit proteins were detected and identified along the mass spectrum. Fifty-five strains of M. aeruginosa from different habitats were analyzed using MALDI-TOF MS; among these samples, different ribosomal protein types were observed. A polygenetic analysis was performed using an unweighted pair-group method with arithmetic means and different ribosomal protein types to classify the strains into five major clades. Two clades primarily contained toxic strains, and the other three clades contained exclusively non-toxic strains. This is the first study to differentiate cyanobacterial strains using MALDI-TOF MS.
Volume 11(5)
Pages e0156275
Published 2016-1-1
DOI 10.1371/journal.pone.0156275
PII PONE-D-16-08617
PMID 27227555
PMC PMC4881969
MeSH Microcystis / classification Microcystis / genetics* Microcystis / metabolism* Models, Genetic* Phylogeny Ribosomal Proteins / genetics* Ribosomal Proteins / metabolism* Ribosome Subunits / genetics Ribosome Subunits / metabolism* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 2.74
Times Cited 5
WOS Category MICROBIOLOGY
Resource
Algae NIES-44 NIES-87 NIES-88 NIES-89 NIES-90 NIES-100 NIES-101 NIES-104 NIES-105 NIES-107 NIES-109 NIES-111 NIES-112 NIES-478 NIES-604 NIES-843 NIES-901 NIES-1043 NIES-1053 NIES-1058 NIES-1061 NIES-1068 NIES-1069 NIES-1070 NIES-1072 NIES-1076 NIES-1077 NIES-1085 NIES-1094 NIES-1099 NIES-1101 NIES-1104 NIES-1105 NIES-1113 NIES-1115 NIES-1126 NIES-1134 NIES-1135 NIES-1144 NIES-1151 NIES-1157 NIES-1170 NIES-1211 NIES-1213 NIES-1215 NIES-1216 NIES-1217 NIES-1218 NIES-1222 NIES-1224 NIES-1230 NIES-1232 NIES-1233 NIES-1239