Reference - Detail
| RRC ID | 37473 |
|---|---|
| Author | Oda T, Nakamura A, Shikayama M, Kawano I, Ishimatsu A, Muramatsu T. |
| Title | Generation of reactive oxygen species by raphidophycean phytoplankton. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Chattonella marina, a raphidophycean flagellate, is one of the most toxic red tide phytoplankton and causes severe damage to fish farming. Recent studies demonstrated that Chattonella sp. generates superoxide (O2-), hydrogen peroxide (H2O2), and hydroxyl radicals (.OH), which may be responsible for the toxicity of C. marina. In this study, we found the other raphidophycean flagellates such as Heterosigma akashiwo, Olisthodiscus luteus, and Fibrocapsa japonica also produce O2- and H2O2 under normal growth condition. Among the flagellate species tested, Chattonella has the highest rates of production of O2- and H2O2 as compared on the basis of cell number. This seems to be partly due to differences in their cell sizes, since Chattonella is larger than other flagellate species. The generation of O2- by these flagellate species was also confirmed by a chemiluminescence assay by using 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one (MCLA). All these raphidophycean flagellates inhibited the proliferation of a marine bacterium, Vibrio alginolyticus, in a flagellates/bacteria co-culture system, and their toxic effects were suppressed by the addition of superoxide dismutase (SOD) or catalase. Our results suggest that the generation of reactive oxygen species is a common feature of raphidophycean flagellates. |
| Volume | 61(10) |
| Pages | 1658-62 |
| Published | 1997-10-1 |
| DOI | 10.1271/bbb.61.1658 |
| PMID | 9362113 |
| MeSH | Animals Catalase / pharmacology Cell Size Eukaryota / metabolism* Hydrogen Peroxide / metabolism* Hydroxyl Radical / metabolism* Imidazoles / chemistry Luminescent Measurements Microscopy, Phase-Contrast Phytoplankton / metabolism* Pyrazines / chemistry Reactive Oxygen Species* Superoxide Dismutase / metabolism Superoxides / metabolism* |
| IF | 1.516 |
| Times Cited | 118 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Wikipedia |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-6 NIES-15 |