論文 - 詳細
| RRC ID | 37481 |
|---|---|
| 著者 | Hertsenberg AJ, Funderburgh JL. |
| タイトル | Generation of Corneal Keratocytes from Human Embryonic Stem Cells. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Human Embryonic Stem Cells (hESC) offer an important resource as a limitless supply of any differentiated cell type of the human body. Keratocytes, cells from the corneal stroma, may have the potential for restoration of vision in cell therapy and biomedical engineering applications, but these specialized cells are not readily expanded in vitro. Here we describe a two-part method to produce keratocytes from the H1 hESC cell line. The hESC cells, maintained and expanded in feeder-free culture medium are first differentiated to neural crest cells using the stromal-derived inducing activity (SDIA) of the PA6 mouse embryonic fibroblast cell line. The resulting neural crest cells are selected by their expression of cell-surface CD271 and subsequently cultured as 3D pellets in a defined differentiation medium to induce a keratocyte phenotype. |
| 巻・号 | 1341 |
| ページ | 285-94 |
| 公開日 | 2016-1-1 |
| DOI | 10.1007/7651_2015_231 |
| PMID | 26026882 |
| PMC | PMC5334125 |
| MeSH | Animals Cell Culture Techniques / methods* Cell Differentiation Cell Line Coculture Techniques / methods Corneal Keratocytes / cytology* Human Embryonic Stem Cells / cytology* Humans Mice Neural Crest / cytology* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |