論文 - 詳細
| RRC ID | 37493 |
|---|---|
| 著者 | Imaizumi K, Iha M, Nishishita N, Kawamata S, Nishikawa S, Akuta T. |
| タイトル | A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Protocols available for the cryopreservation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells are very inefficient and laborious compared to those for the cryopreservation of murine ES/iPS cells or other general cell lines. While the vitrification method may be adequate when working with small numbers of human ES/iPS cells, it requires special skills and is unsuitable when working with large cell numbers. Here, we describe a simple and efficient method for the cryopreservation of hES/hiPS cells that is based on a conventional slow freezing method that uses a combination of Pronase/EDTA for Stem™ and CP-5E™ [final concentrations: 6 % hydroxyethyl starch, 5 % DMSO, and 5 % ethylene glycol in saline]. CP-5E™ is highly effective for the cryopreservation of small cell clumps produced by hES/hiPS colony detachment in the presence of Pronase and EDTA (Pronase/EDTA for Stem™, a formulation containing multiple digestive enzymes from Streptomyces griseus). This novel method would be quite useful for large-scale hES/iPS cell banking for use in clinical applications. |
| 巻・号 | 1341 |
| ページ | 15-24 |
| 公開日 | 2016-1-1 |
| DOI | 10.1007/7651_2015_211 |
| PMID | 26069023 |
| MeSH | Cell Culture Techniques / methods Cell Proliferation Cryopreservation / economics Cryopreservation / methods* Cryoprotective Agents / pharmacology* Dimethyl Sulfoxide / pharmacology Ethylene Glycol / pharmacology Freezing Human Embryonic Stem Cells / cytology* Human Embryonic Stem Cells / drug effects Humans Hydroxyethyl Starch Derivatives / pharmacology Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects Pronase / pharmacology Staining and Labeling / methods Streptomyces griseus / enzymology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) KhES-1(HES0001) |