RRC ID 37498
著者 Ichimura T, Chiu LD, Fujita K, Machiyama H, Kawata S, Watanabe TM, Fujita H.
タイトル Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.
ジャーナル Sci Rep
Abstract Using Raman spectral imaging, we visualized the cell state transition during differentiation and constructed hypothetical potential landscapes for attractors of cellular states on a state space composed of parameters related to the shape of the Raman spectra. As models of differentiation, we used the myogenic C2C12 cell line and mouse embryonic stem cells. Raman spectral imaging can validate the amounts and locations of multiple cellular components that describe the cell state such as proteins, nucleic acids, and lipids; thus, it can report the state of a single cell. Herein, we visualized the cell state transition during differentiation using Raman spectral imaging of cell nuclei in combination with principal component analysis. During differentiation, cell populations with a seemingly homogeneous cell state before differentiation showed heterogeneity at the early stage of differentiation. At later differentiation stages, the cells returned to a homogeneous cell state that was different from the undifferentiated state. Thus, Raman spectral imaging enables us to illustrate the disappearance and reappearance of an attractor in a differentiation landscape, where cells stochastically fluctuate between states at the early stage of differentiation.
巻・号 5
ページ 11358
公開日 2015-6-16
DOI 10.1038/srep11358
PII srep11358
PMID 26079396
PMC PMC5155549
MeSH Animals Cell Differentiation* Cell Line Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism Mice Muscle Development Myoblasts / cytology Myoblasts / metabolism Spectrum Analysis, Raman*
IF 3.998
引用数 14
WOS 分野 MULTIDISCIPLINARY SCIENCES
リソース情報
ヒト・動物細胞 C2C12(RCB0987)