論文 - 詳細
| RRC ID | 37502 |
|---|---|
| 著者 | Niikura K, Horisawa K, Doi N. |
| タイトル | A fusogenic peptide from a sea urchin fertilization protein promotes intracellular delivery of biomacromolecules by facilitating endosomal escape. |
| ジャーナル | J Control Release |
| Abstract |
The low efficiency of endosomal escape has been considered a bottleneck for the cytosolic delivery of biomacromolecules such as proteins and DNA. Although fusogenic peptides (FPs) such as HA2 have been employed to improve the intracellular delivery of biomacromolecules, the FPs studied thus far are not adequately efficient in enabling endosomal escape; therefore, novel FPs with higher activity are required. In this context, we focused on FPs derived from a sea urchin fertilization protein, bindin, which is involved in gamete recognition (B18, residues 103-120 and B55, residues 83-137 of mature bindin). We show that enhanced green fluorescent protein (EGFP)-fused B55 peptide binds to plasma membranes more strongly than EGFP-B18 and promotes the intracellular delivery of dextrans, which were co-administered using the trans method in a pH-dependent manner without affecting cell viability and proliferation, whereas conventional EGFP-HA2 did not affect dextran internalization. Furthermore, EGFP-B55 promoted the intracellular delivery of biomacromolecules such as antibodies, ribonuclease and plasmidic DNA using the trans method. Because the promotion of intracellular delivery by EGFP-B55 was suppressed by endocytosis inhibitors, EGFP-B55 is considered to have facilitated the endosomal escape of co-administered cargos. These results suggested that an FP that promotes the intracellular delivery of a variety of biomacromolecules with no detectable cytotoxicity should be useful for the cytosolic delivery of membrane-impermeable molecules for biomedical and biotechnological applications. |
| 巻・号 | 212 |
| ページ | 85-93 |
| 公開日 | 2015-8-28 |
| DOI | 10.1016/j.jconrel.2015.06.020 |
| PII | S0168-3659(15)00627-6 |
| PMID | 26091921 |
| MeSH | Cell Survival / drug effects DNA / administration & dosage DNA / chemistry Dextrans / administration & dosage Dextrans / chemistry Drug Delivery Systems* Endocytosis Endosomes / metabolism Fertilization Green Fluorescent Proteins / chemistry* HeLa Cells Humans Immunoglobulin G / administration & dosage Immunoglobulin G / chemistry Luciferases / genetics Peptides / chemistry* Receptors, Cell Surface / chemistry* Ribonucleases / administration & dosage Ribonucleases / chemistry |
| IF | 7.727 |
| 引用数 | 9 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |