Reference - Detail
|Author||Motoyama K, Sako A, Ibrahim Abu Hashim I, Higashi T, Arima H.|
|Title||Effects of dendrimer/cyclodextrin conjugates as gene transfer carriers on nitric oxide production from macrophages.|
|Journal||J. Pharm. Pharmacol.|
OBJECTIVES:The development of safe gene transfer carriers with high transfection efficiency, which does not affect the cell function, is a challenging issue. In this study, we examined the effects of α-cyclodextrin (α-CyD)/dendrimer conjugate (α-CDE (G3)) on nitric oxide (NO) production in murine macrophages J774.1 cells stimulated with toll-like receptors (TLR) ligands.
METHODS:NO production from macrophages stimulated with TLR ligands was determined by the Griess method. Transfection efficiency of α-CDE (G3)/plasmid DNA (pDNA) complex was quantified by a luminometer.
KEY FINDINGS:α-CDE (G3) significantly inhibited NO production from J774.1 cells stimulated with TLR ligands. α-CyD molecules in α-CDE (G3) had no effect on NO production. The inhibitory effect of α-CDE (G3) on NO production might be attributed to the dendrimer (G3). Increasing the degree of substitution (DS) of α-CyD in the α-CDE (G3) molecule was accompanied by a significant decrease in the inhibition of NO production. Furthermore, higher gene transfection efficiency of α-CDE (G3)/pDNA complex was observed upon increasing the DS of α-CyD.
CONCLUSIONS:α-CDE (G3) with high DS value of α-CyD may be considered as a safe gene transfer carrier that does not adversely affect NO production from macrophages stimulated with TLR ligands.
|MeSH||Animals Cell Line Cell Survival / drug effects Dendrimers / chemistry Dendrimers / metabolism Dendrimers / toxicity* Dose-Response Relationship, Drug Ligands Macrophages / drug effects* Macrophages / metabolism Mice Nitric Oxide / metabolism* Nitrites / metabolism Toll-Like Receptors / agonists Toll-Like Receptors / metabolism Transfection / methods* alpha-Cyclodextrins / chemistry alpha-Cyclodextrins / metabolism alpha-Cyclodextrins / toxicity*|
|WOS Category||PHARMACOLOGY & PHARMACY|
|Human and Animal Cells||J774.1(RCB0434)|