| RRC ID |
37550
|
| 著者 |
Ninomiya K, Yamashita T, Tanabe Y, Imai M, Takahashi K, Shimizu N.
|
| タイトル |
Targeted and ultrasound-triggered cancer cell injury using perfluorocarbon emulsion-loaded liposomes endowed with cancer cell-targeting and fusogenic capabilities.
|
| ジャーナル |
Ultrason Sonochem
|
| Abstract |
This study investigated the targeting and ultrasound-triggered injury of cancer cells using anticancer drug-free liposomes that contained an emulsion of perfluoropentane (ePFC5) and were co-modified with avidin as a targeting ligand for cancer cells and the hemagglutinating virus of Japan (HVJ) envelope to promote liposome fusion with the cells. These liposomes are designated as ePFC5-loaded avidin/HVJ liposomes. ePFC5-loaded liposomes were sensitized to ultrasound irradiation. Liposomes modified with avidin alone (avidin liposomes) showed binding to MCF-7 human breast cancer cells, and liposomes modified with HVJ envelope alone (HVJ liposomes) were found to fuse with MCF-7 cells. The irradiation of MCF-7 cells with 1 MHz ultrasound (30s, 1.2 W/cm(2), duty ratio 30%) combined with ePFC5-loaded avidin/HVJ liposomes resulted in a decrease in cell viability at 1h after irradiation to 43% of that of controls without ultrasound irradiation or liposomes. The cell viability was lower than that of cells treated with ultrasound irradiation with ePFC5-loaded avidin liposomes or ePFC5-loaded HVJ liposomes. This indicates that co-modification of liposome with avidin and HVJ envelope could enhance ultrasound-induced cell injury in the presence of ePFC5-loaded liposomes.
|
| 巻・号 |
28
|
| ページ |
54-61
|
| 公開日 |
2016-1-1
|
| DOI |
10.1016/j.ultsonch.2015.06.032
|
| PII |
S1350-4177(15)00204-7
|
| PMID |
26384883
|
| MeSH |
Avidin / metabolism
Biological Transport
Cell Survival / drug effects
Cytosol / metabolism
Emulsions
Fluorocarbons / administration & dosage*
Fluorocarbons / chemistry
Fluorocarbons / metabolism
Fluorocarbons / pharmacology*
Humans
Liposomes
MCF-7 Cells
Sendai virus / metabolism
Ultrasonic Waves*
Volatilization
|
| IF |
6.513
|
| 引用数 |
12
|
|
WOS 分野
|
ACOUSTICS
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ヒト・動物細胞 |
MCF7(RCB1904) |
