RRC ID 37558
著者 Cho SH, Du J, Sines I, Poosarla VG, Vepachedu V, Kafle K, Park YB, Kim SH, Kumar M, Nixon BT.
タイトル In vitro synthesis of cellulose microfibrils by a membrane protein from protoplasts of the non-vascular plant Physcomitrella patens.
ジャーナル Biochem J
Abstract Plant cellulose synthases (CesAs) form a family of membrane proteins that are associated with hexagonal structures in the plasma membrane called CesA complexes (CSCs). It has been difficult to purify plant CesA proteins for biochemical and structural studies. We describe CesA activity in a membrane protein preparation isolated from protoplasts of Physcomitrella patens overexpressing haemagglutinin (HA)-tagged PpCesA5. Incubating the membrane preparation with UDP-glucose predominantly produced cellulose. Negative-stain EM revealed microfibrils. Cellulase bound to and degraded these microfibrils. Vibrational sum frequency generation (SFG) spectroscopic analysis detected the presence of crystalline cellulose in the microfibrils. Putative CesA proteins were frequently observed attached to the microfibril ends. Combined cross-linking and gradient centrifugation showed bundles of cellulose microfibrils with larger particle aggregates, possibly CSCs. These results suggest that P. patens is a useful model system for biochemical and structural characterization of plant CSCs and their components.
巻・号 470(2)
ページ 195-205
公開日 2015-9-1
DOI 10.1042/BJ20141391
PII BJ20141391
PMID 26348908
MeSH Bryopsida / chemistry* Cellulose / chemistry* Glucosyltransferases / chemistry* Membrane Proteins / chemistry* Microfibrils / chemistry* Plant Lectins / chemistry Plant Proteins / chemistry* Protoplasts / chemistry*
IF 4.097
引用数 8
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pdp24095