| RRC ID |
37582
|
| 著者 |
Miura R, Kooriyama T, Yoneda M, Takenaka A, Doki M, Goto Y, Sanjoba C, Endo Y, Fujiyuki T, Sugai A, Tsukiyama-Kohara K, Matsumoto Y, Sato H, Kai C.
|
| タイトル |
Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs.
|
| ジャーナル |
PLoS Negl Trop Dis
|
| Abstract |
Canine distemper virus (CDV) vaccination confers long-term protection against CDV reinfection. To investigate the utility of CDV as a polyvalent vaccine vector for Leishmania, we generated recombinant CDVs, based on an avirulent Yanaka strain, that expressed Leishmania antigens: LACK, TSA, or LmSTI1 (rCDV-LACK, rCDV-TSA, and rCDV-LmSTI1, respectively). Dogs immunized with rCDV-LACK were protected against challenge with lethal doses of virulent CDV, in the same way as the parental Yanaka strain. To evaluate the protective effects of the recombinant CDVs against cutaneous leishmaniasis in dogs, dogs were immunized with one recombinant CDV or a cocktail of three recombinant CDVs, before intradermal challenge (in the ears) with infective-stage promastigotes of Leishmania major. Unvaccinated dogs showed increased nodules with ulcer formation after 3 weeks, whereas dogs immunized with rCDV-LACK showed markedly smaller nodules without ulceration. Although the rCDV-TSA- and rCDV-LmSTI1-immunized dogs showed little protection against L. major, the cocktail of three recombinant CDVs more effectively suppressed the progression of nodule formation than immunization with rCDV-LACK alone. These results indicate that recombinant CDV is suitable for use as a polyvalent live attenuated vaccine for protection against both CDV and L. major infections in dogs.
|
| 巻・号 |
9(7)
|
| ページ |
e0003914
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pntd.0003914
|
| PII |
PNTD-D-15-00333
|
| PMID |
26162094
|
| PMC |
PMC4498809
|
| MeSH |
Animals
Antibodies, Viral / immunology
Antigens, Protozoan / administration & dosage
Antigens, Protozoan / genetics
Antigens, Protozoan / immunology*
Distemper Virus, Canine / genetics*
Distemper Virus, Canine / metabolism
Dogs
Female
Genetic Vectors / genetics
Genetic Vectors / metabolism
Humans
Leishmania major / genetics
Leishmania major / immunology*
Leishmaniasis, Cutaneous / immunology*
Leishmaniasis, Cutaneous / parasitology
Leishmaniasis, Cutaneous / prevention & control
Protozoan Vaccines / administration & dosage
Protozoan Vaccines / genetics
Protozoan Vaccines / immunology*
Viral Vaccines / administration & dosage*
Viral Vaccines / genetics
Viral Vaccines / metabolism
|
| IF |
3.885
|
| 引用数 |
11
|
|
WOS 分野
|
TROPICAL MEDICINE
PARASITOLOGY
INFECTIOUS DISEASES
|
| リソース情報 |
| ヒト・動物細胞 |
293(RCB1637) |
